Item Type |
Article |
ID |
|
Preview |
Image |
|
Caption |
|
|
Full text |
KAKEN_24700986seika.pdf
Type |
:application/pdf |
Download
|
Size |
:389.1 KB
|
Last updated |
:Dec 11, 2014 |
Downloads |
: 981 |
Total downloads since Dec 11, 2014 : 981
|
|
Release Date |
|
Title |
Title |
グリオブラストーマの腫瘍形成規定因子の同定
|
Kana |
グリオブラストーマ ノ シュヨウ ケイセイ キテイ インシ ノ ドウテイ
|
Romanization |
Gurioburasutoma no shuyo keisei kitei inshi no dotei
|
|
Other Title |
Title |
Identification of key factors of glioblastoma tumorigenesis
|
Kana |
|
Romanization |
|
|
Creator |
Name |
Sampetrean, Oltea
|
Kana |
サンペトラ, オルテア
|
Romanization |
|
Affiliation |
慶應義塾大学・医学部・特任助教
|
Affiliation (Translated) |
|
Role |
Research team head
|
Link |
科研費研究者番号 : 50571113
|
|
Edition |
|
Place |
|
Publisher |
|
Date |
Issued (from:yyyy) |
2014
|
Issued (to:yyyy) |
|
Created (yyyy-mm-dd) |
|
Updated (yyyy-mm-dd) |
|
Captured (yyyy-mm-dd) |
|
|
Physical description |
|
Source Title |
Name |
科学研究費補助金研究成果報告書
|
Name (Translated) |
|
Volume |
|
Issue |
|
Year |
2013
|
Month |
|
Start page |
|
End page |
|
|
ISSN |
|
ISBN |
|
DOI |
|
URI |
|
JaLCDOI |
|
NII Article ID |
|
Ichushi ID |
|
Other ID |
|
Doctoral dissertation |
Dissertation Number |
|
Date of granted |
|
Degree name |
|
Degree grantor |
|
|
Abstract |
グリオブラストーマ起源細胞の特性を解析し、腫瘍塊形成を規定する因子の同定を目標として研究を行った。具体的には : (1)グリオブラストーマのマウスモデルを用いて、複数の腫瘍起源細胞のクローンを樹立し、その増殖能、浸潤能などのフェノタイプ解析を行った。その結果、腫瘍起源細胞の代謝特性がクローンによって大きく異なることを見出した。(2)樹立した腫瘍起源細胞の遺伝子プロファイルを解析し、(1)で同定した性質の規定因子候補を同定した。(3)同定した特性・規定因子の機能解析を行い、グリオブラストーマ起源細胞がエネルギー代謝経路として解糖経路のみならず、酸化的リン酸化経路も使用できることを証明した。
The goal of this study was to investigate the characteristics of glioma-initiating cells (GICs) and identify key factors regulating the formation of glioblastoma. Using our syngeneic murine model, we conducted research as follows.
(1) We established several GIC clones and analyzed their basic properties, such as proliferation, invasion and ability to induce microvascular proliferation. As a result, we found that metabolic characteristics such as glucose consumption and lactic acid production can differ significantly between clones. (2) We analyzed the gene expression profile of our GIC clones and identified factors involved in the regulation of their metabolic characteristics. (3) We performed a functional analysis of the data obtained in (1), (2) and showed that GICs can use not only glycolysis, but also oxidative phosphorylation during glioblastoma formation.
|
|
Table of contents |
|
Keyword |
|
NDC |
|
Note |
研究種目 : 若手研究(B)
研究期間 : 2012~2013
課題番号 : 24700986
研究分野 : 総合領域
科研費の分科・細目 : 腫瘍学・腫瘍生物学
|
|
Language |
|
Type of resource |
|
Genre |
|
Text version |
|
Related DOI |
|
Access conditions |
|
Last modified date |
|
Creation date |
|
Registerd by |
|
History |
|
Index |
|
Related to |
|