マウス子宮内膜上皮細胞 (EEC) では、CD9が発情期と発情間期は基底膜側に局在し、着床期では子宮内腔側に局在し胚の着床部位に一致した。CD9欠損マウスのECCでは発情期に微絨毛が短小化し、ミトコンドリア数は有意に減少した。ECCにおけるCD9の再配置と細胞外分泌がECC膜構造に影響し、内膜の着床能を制御すると考えられた。
ヒト胚盤胞培養後培養液中のエクソソームに含まれるmiRNAについて患者同意のもと解析した。胚から分泌される8つのmiRNAを見出し、ロジスティック回帰モデル (5-fold Cross Validationで平均 Accuracy0.82) により胚の妊娠率予測が可能であった。
Tetraspanin membrane protein CD9 was localized predominantly on the basal region of uterine epithelial cells at estrus-stage mice, but relocated to the apical region upon embryo implantation. Extracellular CD9 was most detected in uterine cavity at the estrus stage. In uterine epithelial cells lacking Cd9 genes, membrane projections were shortened and the number of mitochondria was reduced. CD9 repositioning and release may affect both membrane structures and mitochondrial state in the uterus, and contribute to female fertility.
miRNAs secreted via exosome from human blastocysts were profiled using RNA-seq and qRT-PCR. The spent medium in which a good-qualty blastocyst was harvested was analyzed. Some of miRNAs were significantly highly expressed in the spent media derived from blastocysts which resulted in implantation failure, compared to those in pregnancy. The embryo selection based on the detection of the specific miRNAs in spent medium may improve the pregnancy rate.

研究種目 : 基盤研究 (B) (一般)
研究期間 : 2016～2019
課題番号 : 16H05475
研究分野 : 生殖医療