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KAKEN_24310148seika.pdf
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Download
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:500.7 KB
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:Apr 11, 2016 |
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| Title |
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mRNAと新生ペプチドの局所構造による翻訳効率変化のゲノムワイド解析
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mRNA ト シンセイ ペプチド ノ キョクショ コウゾウ ニ ヨル ホンヤク コウリツ ヘンカ ノ ゲノム ワイド カイセキ
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mRNA to shinsei pepuchido no kyokusho kozo ni yoru honyaku koritsu henka no genomu waido kaiseki
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Genome wide analysis of translation efficiency by local structure of mRNA and nascent peptide
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中東, 憲治
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ナカヒガシ, ケンジ
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Nakahigashi, Kenji
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慶應義塾大学・政策･メディア研究科・特任准教授
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Research team head
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科研費研究者番号 : 70322740
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森, 浩禎
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モリ, ヒロタダ
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Mori, Hirotada
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奈良先端科学技術大学院大学・バイオサイエンス研究科・教授
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Research team member
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科研費研究者番号 : 90182203
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金井, 昭夫
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カナイ, アキオ
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Kanai, Akio
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慶應義塾大学･環境情報学部･教授
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Research team member
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科研費研究者番号 : 60260329
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2015
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科学研究費補助金研究成果報告書
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2014
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| Abstract |
遺伝子mRNAからタンパク質が翻訳される状況を新しい実験手法で解析し, ゲノム全体の遺伝子を見た場合, 翻訳開始の構造が翻訳の効率に大きな影響を与えることを明らかにした。対して, 翻訳伸長の効率にも差はあったが, 遺伝子全体の翻訳効率に大きな影響は与えていなかった。また, コドン-アンチコドンの3文字目の認識や, small RNA/Hfqによる翻訳制御の実体など, ネイティブな翻訳関連因子の性質についてや, 様々な抗生物質の作用機序など, これまで知られていなかった性質を示す新たな知見を得た。
We performed genome wide analysis of efficiency in mRNA translation to protein, employing new method. We experimentally showed that rate of initiation largely affected the overall efficiency of a gene, while difference in elongation efficiency by codon usage did exist but the effect was much smaller.
We also found new insights in the nature of translation apparatus and factors, such as efficiency of non-canonical codon-anticodon recognition at 3rd letter and translational regulation by sRNA/Hfq, as well as effect of nascent-peptide sequence on specificity of various antibiotics.
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研究種目 : 基盤研究(B)
研究期間 : 2012～2014
課題番号 : 24310148
研究分野 : システム生物学
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