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KAKEN_24792152seika.pdf
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Download
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:276.1 KB
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Total downloads since Sep 1, 2023 : 836
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| Title |
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超急速凍結とアクアポリン発現による幹細胞の選択・濃縮
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チョウキュウソク トウケツ ト アクアポリン ハツゲン ニ ヨル カンサイボウ ノ センタク・ノウシュク
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Chokyusoku toketsu to akuaporin hatsugen ni yoru kansaibo no sentaku noshuku
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Cryopreservation for the cell selection using water channels AQPs combination with ultra-quick freezing
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加藤, 靖浩
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カトウ, ヤスヒロ
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Kato, Yasuhiro
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慶應義塾大学・医学部・助教
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Research team head
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科研費研究者番号 : 40398780
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2015
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科学研究費補助金研究成果報告書
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2014
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本研究によって, 耐凍性を獲得したアクアポリン発現細胞のみ超急速凍結(液体窒素)によって選択されることを明らかにした(PLOS ONE [Feb 2014, vol19]。つまり, AQP4発現による耐凍性をマーカーとした新しい凍結方法を確立することで, 癌化が危惧される幹細胞の排除ならびに雑多な細胞集団から特定のアクアポリン細胞のみを選択できるシンプルな方法である。この方法は, 従来のマーカー染色やソーティング作業を必要としない。
Here we show that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4. We next examine if the expression of AQP4 combined with ultra-quick freezing can bes used to select cells efficiently under freezing conditions where most cells are died. Finally we propose that this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in regenerative medicine as well as cell-based functional assays or drug screening protocols.
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研究種目 : 若手研究(B)
研究期間 : 2012～2014
課題番号 : 24792152
研究分野 : 生化学
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| Sep 1, 2023 | | Creator,Abstract 内容,Note 注記 を変更 |
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