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KAKEN_25860621seika  
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Title 心筋分化における長鎖non-coding RNAの制御機構の解明  
Kana シンキン ブンカ ニ オケル チョウサ non-coding RNA ノ セイギョ キコウ ノ カイメイ  
Romanization Shinkin bunka ni okeru chosa non-coding RNA no seigyo kiko no kaimei  
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Title Long non coding RNAs regulate the cardiac lineage during embryonic stem cell differentiation  
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Name 西山, 崇比古  
Kana ニシヤマ, タカヒコ  
Romanization Nishiyama, Takahiko  
Affiliation 慶應義塾大学・医学部・特任助教  
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Role Research team head  
Link 科研費研究者番号 : 20464844
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Issued (from:yyyy) 2016  
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1 pdf  
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Name 科学研究費補助金研究成果報告書  
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Year 2015  
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Abstract
本研究では, non coding RNAでもまだ機能が十分に解明されていないlinc RNAに着目した。心臓発生と心筋分化誘導下において必要なlinc RNAを同定するためにマウス胎児, 出生後, 成獣からRNAを抽出した。また, ES細胞から心筋分化誘導し作成した心筋細胞と未分化状態のES細胞からRNAを抽出した。これらの, サンプルをnon coding RNA arrayを行い, 発生段階に発現量の変化を認めた心臓発生に必要と考えられるlinc RNAを特定することができた。また, RT-PCRにより同lincRNAの発現量がarrayと一致することを確認した。
Long non coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides, but they do not code proteins. LncRNAs constitute a heterogenic class of RNAs that includes intergenic RNAs, antisense transcripts, and enhancer RNAs. In recent, several studies have been reported that lncRNAs required for cardiac development and disease. It is obvious that many lncRNAs are expressed in cardiac development and are necessary for cardiovascular lineage. However, it has not been investigated in detail how lncRNAs are regulated in cardiac development and heart failure.Using embryonic stem cell (ESC) differentiation strategies, lncRNAs which restricted to cardiac cell were analyzed. In this study, to establish a map of lncRNAs expression and potential target gene networks during ESC differentiation. We identified the potential target genes of these lncRNAs through a bioinformatic correlation network that clusters lncRNAs into specific functional pathway.
 
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Keyword
long non coding RNA  
NDC
 
Note
研究種目 : 若手研究(B)
研究期間 : 2013~2015
課題番号 : 25860621
研究分野 : 循環器内科
 
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日本語  

英語  
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Research Paper  
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Dec 27, 2016 11:19:03  
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Dec 27, 2016 11:19:03  
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/ Public / Grants-in-Aid for Scientific Research / Fiscal year 2015 / Japan Society for the Promotion of Science
 
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