本研究では、neutrophil gelatinase-associated lipocalin (NGAL)の細菌鉄獲得阻害機能(静菌効果)に着目し、臨床的に問題となる感染症起因菌に対するNGALの静菌効果を検討するため臨床検体より高頻度に単離されるグラム陰性菌の同定調査を行い、大腸菌(E.coli)、肺炎桿菌、エンテロバクター・クロアカエの上位3菌種を同定した。細菌のTuf遺伝子を標的としたreal-time PCR法では、NGAL15μgの添加でE.coliの3時間後の増殖が抑制される傾向を認め、高濃度NGALがE. coliに対して増殖能を抑制する可能性が示唆された。
We previously focused on bacteriostatic effects of neutrophil gelatinase-associated lipocalin (NGAL), known as a biomarker of acute kidney injury, and investigated the interaction between this protein and intestinal microflora. The present study demonstrated that bacteriostatic effects of NGAL against bacteria, which is frequently found in clinical situations. As a result, gram-negative bacteria such as Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae was investigated to examine the bacteriostatic effects of NGAL in vitro. To detect bacterial growth, real-time PCR by using bacteria specific primer was performed. Although 1 mcg NGAL did not prevent the growth of these bacteria in vitro, 15 mcg NGAL tended to inhibit the growth of Escherichia coli, but it did not reach statistically significance. In conclusion, the present study demonstrated that high concentration NGAL might be able to inhibit the growth of Escherichia coli.

研究種目 : 挑戦的萌芽研究
研究期間 : 2012～2013
課題番号 : 24659801
研究分野 : 医歯薬学
科研費の分科・細目 : 外科系臨床医学・救急医学