本研究では, non coding RNAでもまだ機能が十分に解明されていないlinc RNAに着目した。心臓発生と心筋分化誘導下において必要なlinc RNAを同定するためにマウス胎児, 出生後, 成獣からRNAを抽出した。また, ES細胞から心筋分化誘導し作成した心筋細胞と未分化状態のES細胞からRNAを抽出した。これらの, サンプルをnon coding RNA arrayを行い, 発生段階に発現量の変化を認めた心臓発生に必要と考えられるlinc RNAを特定することができた。また, RT-PCRにより同lincRNAの発現量がarrayと一致することを確認した。
Long non coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides, but they do not code proteins. LncRNAs constitute a heterogenic class of RNAs that includes intergenic RNAs, antisense transcripts, and enhancer RNAs. In recent, several studies have been reported that lncRNAs required for cardiac development and disease.
It is obvious that many lncRNAs are expressed in cardiac development and are necessary for cardiovascular lineage. However, it has not been investigated in detail how lncRNAs are regulated in cardiac development and heart failure.Using embryonic stem cell (ESC) differentiation strategies, lncRNAs which restricted to cardiac cell were analyzed. In this study, to establish a map of lncRNAs expression and potential target gene networks during ESC differentiation. We identified the potential target genes of these lncRNAs through a bioinformatic correlation network that clusters lncRNAs into specific functional pathway.
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