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KAKEN_18K13517seika.pdf
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Download
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:1.7 MB
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| Last updated |
:Mar 5, 2021 |
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: 464 |
Total downloads since Mar 5, 2021 : 464
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| Title |
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アクアポリン6のアニオン透過メカニズムの解明
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| Kana |
アクアポリン 6 ノ アニオン トウカ メカニズム ノ カイメイ
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Akuaporin 6 no anion tōka mekanizumu no kaimei
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Anion permeation mechanism of aquaporin 6
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山本, 詠士
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ヤマモト, エイジ
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Yamamoto, Eiji
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| Affiliation |
慶應義塾大学・理工学部 (矢上) ・助教
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Research team head
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科研費研究者番号 : 00779340
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2020
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科学研究費補助金研究成果報告書
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2019
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膜貫通タンパク質であるアクアポリンによる分子の選択的透過機構を分子レベルで明らかにするために，分子動力学シミュレーションを行った．アニオンチャネルとして知られるアクアポリン6は実験による立体構造の報告がないため，タンパク質立体構造予測法によって構造をモデル化した．モデル化したタンパク質構造の生体膜中における安定性をシミュレーションにより確認した．さらに，塩化物イオンの透過経路を特定し，イオン透過時の透過経路内への水分子の充填が重要であることを分子レベルで明らかにした．
Molecular dynamics simulations were performed to elucidate the molecular mechanism of selective permeation of molecules through aquaporins. The structure model of aquaporin 6, which is known as an anion channel, was predicted by a homology modeling because its structure has not been reported experimentally. The stability of the predicted protein conformation in a biological membrane was confirmed by simulations. Furthermore, we clarified the permeation pathway of chloride ions in the aquaporin 6, and that the packing of water molecules into the permeation pathway is important for the ion permeation event.
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研究種目 : 若手研究
研究期間 : 2018～2019
課題番号 : 18K13517
研究分野 : 生物物理
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