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KAKEN_19K22544seika.pdf
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Download
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:179.2 KB
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:Nov 14, 2022 |
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Total downloads since Nov 14, 2022 : 284
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腸内細菌研究のパラダイムシフトを加速する新規オルガノイド培養系の開発
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チョウナイ サイキン ケンキュウ ノ パラダイム シフト オ カソクスル シンキ オルガノイド バイヨウケイ ノ カイハツ
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Chōnai saikin kenkyū no paradaimu shifuto o kasokusuru shinki oruganoido baiyōkei no kaihatsu
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Developing a scalable-coculturing system for gut anaerobes and intestinal epithelium
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佐々木, 伸雄
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ササキ, ノブオ
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Sasaki, Nobuo
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慶應義塾大学・医学部 (信濃町) ・特任助教
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Research team head
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科研費研究者番号 : 30777769
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佐藤, 俊朗
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サトウ, トシロウ
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Satō, Toshirō
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慶應義塾大学・医学部・教授
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Collaborator
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科研費研究者番号 : 70365245
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2021
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科学研究費補助金研究成果報告書
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2020
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本課題研究は，これまで不可能されてきた嫌気性の腸内細菌と好気性のヒト正常腸管上皮細胞を同時に培養する共培養システムの開発を目的とする．初めに申請者らは，３次元組織幹細胞培養法（オルガノイド）を改良し，ボイデンチャンバーを利用した単層培養法によるヒト大腸上皮細胞の長期間培養に成功した．次に，上皮細胞の管腔側のみを嫌気条件にしても大腸上皮が生育できる培地の開発にも取り組み，完成したシステムに偏性嫌気性細菌を培地に添加し培養すると，実際にこれらの細菌は腸管上皮細胞表面で生育コロニーを形成した．このように申請者らは，宿主-細菌間の相互作用の分子基盤を理解するための有益な共培養システムの開発に成功した．
The aim of this study is to develop a novel culturing system of healthy human intestinal epithelium together with anaerobic gut bacteria. We modified the classical culture method for 3D adult-tissue stem cell, organoid firstly. Using the Boyden chamber, we succeeded in culturing human normal colon epithelial cells as the monolayer condition for long periods. Next, we also figure out the suitable conditioned medium to enable to generate anaerobic environments at apical surface of their epithelium. Actually, the obligate anaerobic bacteria can grow to form their colonies on the human colonic epithelial cells in our culture condition. Here, we demonstrated a novel coculturing system in modeling competitive and mutually beneficial relationship between human epithelium and gut microbes in vitro.
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研究種目 : 挑戦的研究 (萌芽)
研究期間 : 2019～2020
課題番号 : 19K22544
研究分野 : 組織幹細胞学
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| May 17, 2022 | | インデックス を変更 |
| Nov 14, 2022 | | Creator Name,Creator Kana,Creator Romanization,Creator Affiliation,Creator Affiliation (Translated),Creator Role,Creator Link,Abstract 内容,Note 注記 を変更 |
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