本研究では, 異物の残らない組織再生型ステントグラフトの開発を試みた。ブタ脱細胞化血管とポリL乳酸を主体とした生体吸収性ステントを縫合固定し, ステントグラフトを作成した。将来的な血管内治療への応用の前段階として, ブタ同種移植術による短期生体適合性評価を行い, 良好な開存性, 耐久性を確認し, さらに従来のステントグラフト被覆材である人工血管と比較して, 良好な内皮化を確認し得た。また, 動脈瘤に対する血管内治療への応用に先立ち, ブタ腹部大動脈への静脈パッチ形成術による形態学的な腹部大動脈瘤モデルを確立した。
The goal of our research is to develop a tissue-engineered stent-graft (TESG) composed of bioresorbable stent and decellularized porcine blood vessels, which is expected to disappear of residual foreign material and integrate with aortic wall over time due to host cell recruitment. The aims of this study are to assess the characteristics of decellularized scaffolds, to create the TESG and to evaluate short term compatibility after implantation in porcine model. To create the TESG, the decellularized porcine vein using our optimized protocol was sutured inside the BRS. Prior to utilization for endovascular treatment, a surgical implantation in porcine model was successfully performed, and the short term results showed good patency and durability of TESG. Furthermore, the area of endothelialization was significantly greater in TESG compare to prosthetic graft. A porcine abdominal aortic aneurysm model was developed for evaluation of TESG in endovascular treatment.
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