| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_26670422seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:80.8 KB
|
| Last updated |
:Sep 21, 2017 |
| Downloads |
: 453 |
Total downloads since Sep 21, 2017 : 453
|
|
| Release Date |
|
| Title |
| Title |
特異的転写因子発現による肺細胞への分化誘導法の確立
|
| Kana |
トクイテキ テンシャ インシ ハツゲン ニ ヨル ハイサイボウ エノ ブンカ ユウドウホウ ノ カクリツ
|
| Romanization |
Tokuiteki tensha inshi hatsugen ni yoru haisaibo eno bunka yudoho no kakuritsu
|
|
| Other Title |
| Title |
Direct reprogramming into pulmonary alveolar epithelial cells by defined transcription factors
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
斎藤, 史武
|
| Kana |
サイトウ, フミタケ
|
| Romanization |
Saito, Fumitake
|
| Affiliation |
慶應義塾大学・医学部・共同研究員
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 30338040
|
| Name |
四倉, 正也
|
| Kana |
ヨツクラ, マサヤ
|
| Romanization |
Yotsukura, Masaya
|
| Affiliation |
慶應義塾大学・医学部・呼吸器外科・助教
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
|
| Name |
石井, 誠
|
| Kana |
イシイ, マコト
|
| Romanization |
Ishii, Makoto
|
| Affiliation |
慶應義塾大学・医学部・講師
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
|
| Name |
家田, 真樹
 |
| Kana |
イエダ, マサキ
|
| Romanization |
Ieda, Masaki
|
| Affiliation |
慶應義塾大学・医学部・講師
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2017
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2016
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
体細胞を起点として幹細胞を経ずに目的の細胞を直接的に誘導する手法(直接リプログラミング法)が, 幹細胞を用いない新たな再生医療の手法として近年報告されている。我々はマウス線維芽細胞に3または4個の特異的因子遺伝子を強制発現させ培養した細胞が, II型肺胞上皮細胞に特異的なマーカーであるSurfactant Protein C(SP-C)を発現していることを免疫染色で確認した。さらにそれらの細胞内にII型肺胞上皮細胞に特異的とされるLamellar bodyの存在を電子顕微鏡で確認した。これらの結果から, 直接リプログラミング法によってII型肺胞上皮細胞が誘導されたことが示唆された。
Direct reprogramming has been reported recently as one of the new methods of regenerative medicine. By direct reprogramming method, somatic cells are converted into other types of cells directly, not using stem cells. We induced 3 or 4 selected factors into fibroblasts of mice. After culturing, the induced cells were found to express Surfactant Protein C, which is a specific marker of type II alveolar cell (AT II) by immunofluorescence staining. The presence of "lamellar body" , which is known as a specific structure of AT II, was detected in the cytoplasm of these induced cells by electron microscopy. These results suggested that induced AT II was obtained by direct reprogramming method.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 挑戦的萌芽研究
研究期間 : 2014～2016
課題番号 : 26670422
研究分野 : 呼吸器内科
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
|
| Index |
|
| Related to |
|
