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KAKEN_26560222seika.pdf
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Download
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:1.2 MB
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:Jan 6, 2017 |
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Total downloads since Jan 6, 2017 : 511
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幹細胞の多殻ビーズ封入技術と完全生体外での毛髪再生への新展開
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カンサイボウ ノ タカク ビーズ フウニュウ ギジュツ ト カンゼン セイタイガイ デノ モウハツ サイセイ エノ シンテンカイ
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Kansaibo no takaku bizu funyu gijutsu to kanzen seitaigai deno mohatsu saisei eno shintenkai
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Hair follicle tissue engineering by fully-in vitro process using multi-layered gel bead culture
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宮田, 昌悟
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ミヤタ, ショウゴ
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Miyata, Shogo
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慶應義塾大学・理工学部・准教授
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Research team head
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科研費研究者番号 : 70376515
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2016
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科学研究費補助金研究成果報告書
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2015
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本研究課題では細胞の空間的配置を制御することで生体外で毛髪再生を実現することにを目的としている。そこで毛包構造を再現するために, 2種の細胞が多層構造を成すコラーゲンゲル培養法の開発を実施した。
具体的には, マウスES細胞を毛乳頭細胞の代替源として第1細胞層とし, その上層をマウス表皮細胞層で覆うコラーゲンゲルビーズの作製法を開発した。同技術を用いて作製した多層ゲルビーズを生体外で培養したところ, 毛包構造およびその周辺組織である立毛筋の再構築を認めた。
以上, 新たに構築した多層ゲルビーズ培養法を用いることでこれまでに実現し得なかった生体外プロセス単独での毛包構造の再生を実現した。
The purpose of this study is to regenerate hair tissue in vitro by controlling three-dimensional cellular organization. To simulate hair follicle structure, collagen gel including two kinds of cells was multi-layered to construct three-dimensional structure.
In this study, mouse embryonic stem cells (mESCs) suspended in collagen gel were micro-spotted and over-layered by mouse fatal epidermal cells suspended in collagen. After the gel beads spotting, cell-seeded collagen gels were cultured in vitro. As the results, hair follicle was regenerated in vitro using our novel culture method. Moreover, arrector pili muscle-like tissue was also regenerated.
In conclusion, three-dimensional culture method simulating hair follicle structure was newly developed. Using our novel method, micro-spotted gel-cultured specimen indicated capability to regenerate follicle by fully-in vitro culture process.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2014～2015
課題番号 : 26560222
研究分野 : 再生医工学
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