iPS細胞を用いた細胞療法に注目が集まっているが, 移植後の拒絶反応や腫瘍形成など問題点が多い。本研究では改良した方法を用いて, 安定した自殺遺伝子導入ヒトiPS細胞を樹立した。神経幹細胞に分化した同細胞をマウス脳内に移植したところ, 脳内に生着し生存した。また薬物投与にて細胞除去する事が出来た。同細胞を悪性脳腫瘍モデル動物に移植したところ, 腫瘍の縮小と生存延長を認めた。以上より, 開発した細胞の治療用幹細胞としての有用性が示された。
Cell therapy using iPS cells is attractive for regenerative medicine, cancer, etc., however, a lot of concerns such as rejection and tumor formation after transplantation remain at moment. To solve those issues, we have established the stable cell line of suicide gene-induced human iPSCs by modified method. When neural stem cells (NSCs) derived from suicide gene-induced human iPSCs were transplanted into mouse brain, those cells were alive and survived well, then wiped out by administration of drug. When those NSCs were transplanted into the brain of malignant brain tumor model animals, the tumor volume reduced and transplanted animals survived longer. Those findings suggested that our established human iPSC line might be useful as a therapeutic stem cells for both stroke and tumor.

研究種目 : 基盤研究(C)(一般)
研究期間 : 2014～2016
課題番号 : 26462171
研究分野 : 脳神経外科, 神経移植