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KAKEN_25430093seika.pdf
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| Title |
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実験用途に応じたNASH肝癌メダカ・マウス・ブタモデルの作成と予防・治療法の開発
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| Kana |
ジッケン ヨウト ニ オウジタ NASH カンガン メダカ・マウス・ブタ モデル ノ サクセイ ト ヨボウ・チリョウホウ ノ カイハツ
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Jikken yoto ni ojita NASH kangan medaka mausu buta moderu no sakusei to yobo chiryoho no kaihatsu
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Development of a novel animal model of hepatocellular carcinoma with nonalcoholic steatohepatitis
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相浦, 浩一
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アイウラ, コウイチ
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Aiura, Koichi
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慶應義塾大学・医学部・講師(非常勤)
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Research team head
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科研費研究者番号 : 00184010
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板野, 理
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イタノ, オサム
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Itano, Osamu
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| Affiliation |
慶應義塾大学・医学部・講師
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Research team member
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科研費研究者番号 : 90265827
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北郷, 実
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キタゴウ, ミノル
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Kitagō, Minoru
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| Affiliation |
慶應義塾大学・医学部・講師
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Research team member
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科研費研究者番号 : 70296599
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松田, 祐子
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マツダ, サチコ
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Matsuda, Sachiko
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| Affiliation |
慶應義塾大学・医学部・特任講師
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Research team member
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科研費研究者番号 : 90534537
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大西, 彰
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オオニシ, アキラ
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Onishi, Akira
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日本大学・生物資源科学部・教授
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Research team member
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科研費研究者番号 : 30414890
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小島, 美咲
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コジマ, ミサキ
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Kojima, Misaki
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独立行政法人農業生物資源研究所・家畜ゲノム研究ユニット・上級研究員
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Research team member
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科研費研究者番号 : 80355742
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谷口, 善仁
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タニグチ, ヨシヒト
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Taniguchi, Yoshihito
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杏林大学・医学部・教授
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Research team member
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科研費研究者番号 : 00324616
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吉浦, 康寿
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ヨシウラ, ヤストシ
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Yoshiura, Yasutoshi
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独立行政法人水産総合研究センター・増養殖研究所・研究員
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Research team member
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科研費研究者番号 : 90372052
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2016
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科学研究費補助金研究成果報告書
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2015
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| Abstract |
本研究では, 非アルコール性脂肪肝炎(NASH)による障害肝からの発癌動物モデルを新規作製した。3週齢のC57BLマウスにHFCD(低コリン脂肪食)を与えると共に, 開始時にper kg 25mgのDENを腹腔内投与した。HFCD群では12週以降でほぼ100%インスリン抵抗性を獲得した。HFCD＋DENは脂肪沈着の他に肝細胞の風船様変性マロリー体などの所見があり, よりNASHに近い組織像が観察され, 腫瘍形成も20週目から確認された。
The aim of this study was to develop a mouse model of hepatocellular carcinoma with nonalcoholic steatohepatitis that develops quickly and reflects all clinically relevant features.
Three-week-old C57BL/6J male mice were fed either a standard diet (MF) or a choline-deficient, high-fat diet (HFCD). The mice in the MF+diethylnitrosamine (DEN) and HFCD+DEN groups received a one-time intraperitoneal injection of DEN at the start of the respective feeding protocols. The mice in the HFCD and HFCD+DEN groups developed obesity early in the experiment and insulin resistance after 12 weeks. The HFCD+DEN group presented the highest incidence of nonalcoholic steatohepatitis. The levels of fibrosis and steatosis varied, but they tended to increase every 4 weeks in the HFCD and HFCD+DEN groups. Computed tomography scans indicated that all the HFCD+DEN mice developed hepatic tumors from 20 weeks, some of which were glutamine synthetase-positive.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2013～2015
課題番号 : 25430093
研究分野 : 外科学
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| Jul 26, 2019 | | 著者,上位タイトル 名前 を変更 |
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