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KAKEN_16H06145seika.pdf
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Download
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:1.5 MB
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:Mar 5, 2021 |
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Total downloads since Mar 5, 2021 : 483
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網羅的な神経伝達物質の可視化法開発と精神疾患研究への適用
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モウラテキナ シンケイ デンタツ ブッシツ ノ カシカホウ カイハツ ト セイシン シッカン ケンキュウ エノ テキヨウ
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Mōratekina shinkei dentatsu busshitsu no kashikahō kaihatsu to seishin shikkan kenkyū eno tekiyō
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Development of simultaneous visualization technology for neurotransmitters and its application to psychiatry disease research
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杉浦, 悠毅
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スギウラ, ユウキ
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Sugiura, Yūki
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慶應義塾大学・医学部 (信濃町) ・講師
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Research team head
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科研費研究者番号 : 30590202
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2020
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科学研究費補助金研究成果報告書
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2019
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これまでに神経伝達物質の局在と代謝の分子実態をダイレクトに捉えられる分子イメージング法は存在しない。そもそも、神経伝達物質の脳内における濃度偏在も未知である。
本研究提案では、情報伝達の「物質実態」である神経伝達物質分子を一斉かつ定量的に局在可視化する高感度な質量分析イメージング法を開発した。さらに、マイクロダイアリシスと質量分析を組み合わせ、放出された伝達物質群の経時的モニタリング法も確立した。これらにより、全脳モノアミン・マッピングによる3次元アトラスを作製し、免疫活性化が引き起こす精神疾患のメカニズムを解明し、さらに疼痛刺激時に応答した側坐核のドパミンの動的な変動を可視化するに至った。
Until now, there is no available molecular imaging technique which is capable of direct visualizations of neurotransmitters and their metabolism. In this research, we developed a highly sensitive mass spectrometry imaging technique that simultaneously and quantitatively visualizes neurotransmitters including monoamines. Furthermore, we have established a method for monitoring the released transmitters in behaving animals by combining microdialysis and mass spectrometry based metabolomics.
By applying these techniques, we generated a three-dimensional monoamine atlas by whole brain monoamine mapping. Moreover, the mechanism of abnormal brain monoamine reduction triggered by immune activation was elucidated. Finally, visualization of the dynamic fluctuation of dopamine in the nucleus accumbens in response to pain stimulation was achieved.
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研究種目 : 若手研究 (A)
研究期間 : 2016～2019
課題番号 : 16H06145
研究分野 : 生化学
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