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KAKEN_15H06587seika.pdf
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アルツハイマー病発症機序の解明を目指す神経細胞特異的解析手法の開発
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アルツハイマービョウ ハツショウ キジョ ノ カイメイ オ メザス シンケイ サイボウ トクイテキ カイセキ シュホウ ノ カイハツ
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Arutsuhaimabyo hatsusho kijo no kaimei o mezasu shinkei saibo tokuiteki kaiseki shuho no kaihatsu
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Development of neuron-specific analytical method to elucidate the mechanism underlying AD pathogenesis
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渡部, 博貴
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ワタナベ, ヒロタカ
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Watanabe, Hirotaka
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慶應義塾大学・医学部・特任講師
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Research team head
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科研費研究者番号 : 30422413
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Shen, Jie
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ハーバード大学・医学部・教授
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Research team member
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2017
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科学研究費補助金研究成果報告書
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2016
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アルツハイマー病発症初期に病理像が出現する成体マウス脳の嗅内野と海馬における神経細胞を単離する手技を検討する。マウス脳組織スライス片から, 実体顕微鏡下で嗅内野第2/3層付近を微小切除し, 神経細胞を含む組織片の酵素処理を行った。神経突起を含む分散した神経細胞をマイクロピペットで拾い, 単一神経細胞cDNAライブラリーの調製を行った。これらのcDNAライブラリーを用いてRT-PCRを行ったところ, 嗅内野の第2/3層特異的なマーカー遺伝子の発現を確認した。
In this research proposal, I aimed to isolate single neuron from entorhinal cortex layer 2/3 of adult mouse, which is most likely vulnerable neuronal population in Alzheimer's disease patients. First, I dissected out entorhinal cortex layer 2/3 from mouse brain slice under stereoscopic microscope, followed by trypsin treatment. Next, I picked single neurons with micropipette, and cDNAs fom each single neuron were synthesized for RT-PCR analysis. Several marker genes specific for entorhinal cortex layer 2/3 can be successfully detected by semiquantitative RT-PCR.
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研究種目 : 研究活動スタート支援
研究期間 : 2015～2016
課題番号 : 15H06587
研究分野 : 神経科学
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| Jul 29, 2019 | | 著者,著者版フラグ を変更 |
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