The purpose of this study was to determine the relationship between the development of motor coordination ability and the amount of daily physical activity in children. Boys (n = 66) and girls (n=65), ranging from 4 to 6 years of age, attending kindergarten and nursery school were considered for this study. The coordination ability (the manual of the motor coordination test was established by the Research Center of Physical Education (RCPE)) and the amount of physical activity were measured. The children were surveyed for a number of steps during consecutive weekdays and a holiday by using Lifecorder (Suzuken Co.). The coordination ability consisted of a jump over and crawl under activity, stick reaction time, open eyes foot balance, and grip strength. Significant correlations were observed between the number of steps per day on the weekdays and that on the holiday for both boys (r=0.466, p0.01) and girls (r = 0.518, p  0.001). Children with a greater number of steps tended to show higher coordination ability; this tendency was observed more clearly in older children. Furthermore, significant correlations were observed between the number of steps per day and the jump over and crawl under activity for boys and girls (p  0.05). These results suggest that the amount of daily physical activity in children have an effect on the development of coordination ability, particularly in older children.