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KAKEN_26670766seika  
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Title
Title 腫瘍幹細胞増殖制御に基づいた横紋筋肉腫治療戦略の開発  
Kana シュヨウ カンサイボウ ゾウショク セイギョ ニ モトズイタ オウモンキン ニクシュ チリョウ センリャク ノ カイハツ  
Romanization Shuyo kansaibo zoshoku seigyo ni motozuita omonkin nikushu chiryo senryaku no kaihatsu  
Other Title
Title Development of treatment strategy for rhabdomyosarcoma based on control of cancer stem cell proliferation  
Kana  
Romanization  
Creator
Name 黒田, 達夫  
Kana クロダ, タツオ  
Romanization Kuroda, Tatsuo  
Affiliation 慶應義塾大学・医学部・教授  
Affiliation (Translated)  
Role Research team head  
Link 科研費研究者番号 : 60170130

Name 檜山, 英三  
Kana ヒヤマ, エイソウ  
Romanization Hiyama, Eiso  
Affiliation 広島大学・学内共同利用施設等・教授  
Affiliation (Translated)  
Role Research team member  
Link 科研費研究者番号 : 00218744

Name 滝田, 順子  
Kana タキタ, ジュンコ  
Romanization Takita, Junko  
Affiliation 東京大学・医学部附属病院・准教授  
Affiliation (Translated)  
Role Research team member  
Link 科研費研究者番号 : 00359621

Name 池田, 均  
Kana イケダ, ヒトシ  
Romanization Ikeda, Hitoshi  
Affiliation 獨協医科大学・医学部・教授  
Affiliation (Translated)  
Role Research team member  
Link 科研費研究者番号 : 10326928

Name 渕本, 康史  
Kana フチモト, ヤスシ  
Romanization Fuchimoto, Yasushi  
Affiliation 慶應義塾大学・医学部・講師(非常勤)  
Affiliation (Translated)  
Role Research team member  
Link 科研費研究者番号 : 40219077

Name 森川, 康英  
Kana モリカワ, ヤスヒデ  
Romanization Morikawa, Yasuhide  
Affiliation 慶應義塾大学・医学部・講師(非常勤)  
Affiliation (Translated)  
Role Research team member  
Link 科研費研究者番号 : 90124958
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Date
Issued (from:yyyy) 2016  
Issued (to:yyyy)  
Created (yyyy-mm-dd)  
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Physical description
1 pdf  
Source Title
Name 科学研究費補助金研究成果報告書  
Name (Translated)  
Volume  
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Year 2015  
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Abstract
PAX3-FKHRまたはPAX7-FKHRキメラ遺伝子は胞巣型・混合型横紋筋肉腫の76%で陽性なのに対し胎児型では検出されず, 生存率はキメラ遺伝子陽性の胞巣型で44.2%に対してキメラ遺伝子陰性の胞巣型では75.0%と有意に高く, 胎児型の76.3%と同様だった。幹細胞マーカー発現は全ての横紋筋肉腫細胞株でCD44陽性, CD44v陰性で, CD133表出率はRh30, KYM-1, RMS-YM株では2%以下, RD株では25%で, CD133陽性細胞は抗癌剤抵抗性で免疫不全マウスにおいて高い造腫瘍能を示した。Genotypeや腫瘍幹細胞のマーカー発現に基づく新治療戦略の可能性が検討された。
Either PAX3-FKHR or PAX7-FKHR gene was positive in 76% of alveolar type rhabomyosarcoma, whereas never detected in embryonal type tumor. Survival rate was significantly lower in alveolar type cases with positive chimeric gene (44.2±7.3%) compared to those with negative chimeric gene (75.0±12.5%), which was similar to that of embryonal cases (76.3±4.9%). ALL mixed type cases were alive regardless of chimeric gene positivity. CD44, a stem cell marker, was positive in all rhabdomyosarcoma cell line examined, whereas, CD44v was negative in all the cell lines. Positivity rate of CD133 was 25% in RD cell line, whereas, less than 2% in other cell lines such as Rh30, KYM-1, RMS-YM. However, CD133 positive cells are chemotherapy resistant, and rapidly formed tumor after administrated in mice. Novel treatment strategy may be stratified according to the genotype and expression of the stem cell makers in rhabdomyosarcoma.
 
Table of contents

 
Keyword
小児外科学  

小児腫瘍学  

小児がん  

腫瘍幹細胞  

横紋筋肉腫  
NDC
 
Note
研究種目 : 挑戦的萌芽研究
研究期間 : 2014~2015
課題番号 : 26670766
研究分野 : 小児外科学
 
Language
日本語  

英語  
Type of resource
text  
Genre
Research Paper  
Text version
publisher  
131.113.194.249
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Last modified date
Dec 27, 2016 11:19:11  
Creation date
Dec 27, 2016 11:19:11  
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History
 
Index
/ Public / Grants-in-Aid for Scientific Research / Fiscal year 2015 / Japan Society for the Promotion of Science
 
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