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KAKEN_26461197seika.pdf
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Title |
Title |
上下気道におけるステロイド抵抗性獲得機序の解明
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Kana |
ジョウゲ キドウ ニ オケル ステロイド テイコウセイ カクトク キジョ ノ カイメイ
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Romanization |
Joge kido ni okeru suteroido teikosei kakutoku kijo no kaimei
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Steroid resistance of upper and lower airways
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福永, 興壱
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フクナガ, コウイチ
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Fukunaga, Koichi
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慶應義塾大学・医学部・専任講師
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Research team head
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科研費研究者番号 : 60327517
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有田, 誠
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アリタ, マコト
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Romanization |
Arita, Makoto
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慶應義塾大学・薬学部・教授
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Research team member
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科研費研究者番号 : 80292952
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茂呂, 和世
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モロ, カズヨ
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Romanization |
Moro, Kazuyo
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国立研究開発法人理化学研究所・総合生命医科学研究センター・チームリーダー
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Research team member
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科研費研究者番号 : 90468489
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2017
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科学研究費補助金研究成果報告書
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2016
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今回申請者は本研究を行うにあたり, 好酸球性副鼻腔炎の手術検体や重症喘息患者末梢血から好酸球, Group2自然リンパ球(ILC2s)を採取し検討した。まず各々の細胞を安定して検体から採取する方法を確立した。特に血液中に数が少ないILC2sに関しては一細胞から解析が可能なSingle cell解析法を用いた。これにより健常者と患者の表現型(増殖能やサイトカイン産生能)の違いを明らかにした。また好酸球に関しても網羅的解析を用いて検討したところ健常者に比べ酵素やメディエーターの産生能の違いがあることを明らかにした。ステロイドの抵抗性についても現在検討を重ねており引き続き研究を行っていく予定である。
In conducting our research regarding steroid resistance of upper and lower airways, we collected eosinophils and Group 2 innate lymphoid cells (ILC 2s) from surgical specimens of chronic eosinophilic sinusitis and peripheral blood of severe asthmatic patients and examined them. First, we established a method to collect each cell stably from the specimen and blood. Especially, ILC2s with small number in blood was examined using single cell analysis method which can analyze from one cell. As a result, the difference between the healthy subjects and the patient's phenotype (proliferative ability and cytokine production capacity) has become clear. In addition, eosinophils were also examined using comprehensive analysis and revealed differences in productivity of enzymes and mediators as compared to healthy subjects. We are currently investigating the resistance of steroids of these cells and will continue to conduct current research.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2014~2016
課題番号 : 26461197
研究分野 : 喘息
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