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KAKEN_26420714seika.pdf
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Download
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:1.1 MB
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:Nov 12, 2018 |
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Total downloads since Nov 12, 2018 : 375
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細胞認識因子を組み込んだインテリジェント型細胞分離基材の開発
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サイボウ ニンシキ インシ オ クミコンダ インテリジェントガタ サイボウ ブンリ キザイ ノ カイハツ
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Saibō ninshiki inshi o kumikonda interijentogata saibō bunri kizai no kaihatsu
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Development of intelligent cell separation materials with cell recognition
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長瀬, 健一
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ナガセ, ケンイチ
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Nagase, Kenichi
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慶應義塾大学・薬学部・准教授
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Research team head
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科研費研究者番号 : 10439838
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2018
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科学研究費補助金研究成果報告書
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2017
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本研究課題では, 再生医療への応用を目的とし, 細胞治療に用いる細胞を温度変化のみで分離するインテリジェント型分離基材の設計を行なった。ガラス基板, 微細加工基板, マイクロファイバーの表面に温度応答性高分子であるポリ(N-イソプロピルアクリルアミド)(PIPAAm)を修飾し, 37℃で細胞を接着させて20℃で細胞を脱着させる基板表面を作製した。この際, 細胞種ごとの接着性の差を大きくする基材設計を行なう事で細胞の分離効率の向上を試みた。
In the present study, thermoresponsive cell separation materials were developed for the application in regenerative medicine. Thermoresponsive polymer, poly (N-isopropylacrylamide), was modified on glass plates, micro structured substrates, and microfibers. Various types of cells were seeded on the prepared substrates at 37℃, and detached at 20℃. Temperature-modulated cell separation was performed using the difference in cell adhesion property on the prepared substrates. Cell separation efficiency using the prepared substrates was improved by increasing the difference in cell adhesion property among cell types.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2014～2017
課題番号 : 26420714
研究分野 : 生体材料
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