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KAKEN_25660256seika.pdf
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:Apr 11, 2016 |
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Total downloads since Apr 11, 2016 : 506
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| Title |
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エピゲノム・バックグラウンドの操作による直接リプログラミング効率の改善
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エピゲノム・バックグラウンド ノ ソウサ ニ ヨル チョクセツ リプログラミング コウリツ ノ カイゼン
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Epigenomu bakkuguraundo no sosa ni yoru chokusetsu ripuroguramingu koritsu no kaizen
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Optimization of direct reprograming methods by understanding the epigenomic features of descendant cell types.
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小田, 真由美
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オダ, マユミ
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Oda, Mayumi
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慶應義塾大学・医学部・助教
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Research team head
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科研費研究者番号 : 80567511
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迫田, 実希
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サコタ, ミキ
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Sakota, Miki
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慶應義塾大学・医学部・研究員
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Research team member
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2015
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科学研究費補助金研究成果報告書
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2014
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本研究計画では, ES細胞を用いた転写因子の強制発現タイミングと異なる培養条件における細胞分化の傾向と効率を確かめることにより, 直接リプログラミングにおける転写因子の役割分担について検討を行った。転写因子強制発現における遺伝子発現変化より, 影響範囲の違いによる転写因子のグループ化を行い検討の材料とした。また, 培養条件による分化効率の違いの検討を各種マーカー遺伝子の発現量の違いにより行い, 時系列を追って発現変化を見ることにより転写因子の役割の違いについて明らかにした。対照として, 各種組織のDNAメチル化プロファイルを作成し, 成熟細胞の持つ特徴的なDNAメチル化パターンを明らかにした。
In this study, we assessed the optimization of direct reprogramming method by analyzing the expression data of transcription factor (TF)-induced ES cell lines and found that the efficiency of cell differentiation examined by several cell type-specific markers was changed according to the role of TFs in direct reprogramming process. We also examined different culture conditions that alters genome-wide epigenetic status, and studied the results of TF-induction under different DNA methylation status. For the comparison, DNA methylation profiles of several tissues were made, which is expected to be used for the understanding the cell type-specific epigenomic patterns and the differences between their descendants.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2013～2014
課題番号 : 25660256
研究分野 : エピゲノム学
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