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KAKEN_25462733seika.pdf
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Download
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:295.8 KB
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:Jan 6, 2017 |
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| Title |
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涙腺における局所ステロイド産生とその生理的役割の解明
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ルイセン ニ オケル キョクショ ステロイド サンセイ ト ソノ セイリテキ ヤクワリ ノ カイメイ
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Ruisen ni okeru kyokusho suteroido sansei to sono seiriteki yakuwari no kaimei
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Locally-produced steroid hormones regulate lacrimal gland function.
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樋口, 明弘
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ヒグチ, アキヒロ
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Higuchi, Akihiro
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慶應義塾大学・医学部・特任講師
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Research team head
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科研費研究者番号 : 20383755
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山崎, 岳
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ヤマザキ, タケシ
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Yamazaki, Takeshi
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広島大学・総合科学研究科・教授
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Research team member
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科研費研究者番号 : 30192397
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向井, 邦晃
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ムカイ, クニアキ
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Mukai, Kuniaki
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慶應義塾大学・医学部・講師
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Research team member
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科研費研究者番号 : 80229913
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2016
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科学研究費補助金研究成果報告書
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2015
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| Abstract |
涙腺にはステロイド合成系が発現しており, 数種類のステロイドホルモンが合成されている。本研究では, 涙腺における局所ステロイド合成酵素の発現解析を行い, 局所ステロイドホルモンの涙腺における生理的役割を検討する。ドライアイモデルラットの涙腺中ステロイド合成酵素の活性を測定したところ, シトクロムP45017α活性の上昇が確認できた。この活性上昇はP45017αおよびシトクロムb5発現の上昇によるものであった。ドライアイモデルでは涙腺中ジヒドロテストステロン濃度が上昇しており, この変動はP45017α活性上昇に起因すると考えられた。涙腺機能は局所ステロイドホルモンによる調節を受けていると思われる。
The lacrimal gland function was affected by steroid hormones. According to the classical theories of endocrinology, steroid hormones are synthesized in specific organs and affect the target tissues. Recent studies showed that locally-synthesized steroid hormones acted on neighboring tissues including themselves in the hippocampus, islets of Langerhans, and musculus skeleti. Expression of steroidogenic enzymes was elucidated by real-time RT-PCR and Immunohistochemical analysis. Activity of steroidogenic enzymes was assayed using LC-MS/MS. In dry eye model rat, the activity of cytochrome P45017α increased accompanied with increase of expressions of P45017α and cytochrome b5 compared with normal rat. LC-MS/MS analysis revealed that dihydrotestosterone (DHT) in lacrimal glands increased in dry eye model rat. Increase of DHT was thought to be caused by increase of P45017α activity. It was considered that the function of lacrimal gland was regulated by locally-produced steroid hormones.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2013～2015
課題番号 : 25462733
研究分野 : 眼生化学
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