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KAKEN_25460487seika.pdf
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Download
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:181.3 KB
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:Jul 30, 2019 |
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Total downloads since Jul 30, 2019 : 220
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ヒト純化細胞を用いた肝多段階発がん過程におけるエピゲノムプロファイル解析
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ヒト ジュンカ サイボウ オ モチイタ カン タダンカイ ハツガン カテイ ニ オケル エピゲノム プロファイル カイセキ
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Hito junka saibo o mochiita kan tadankai hatsugan katei ni okeru epigenomu purofairu kaiseki
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Epigenome profiling of precancerous condition in hepatocarcinogenesis using purified human hepatocytes
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新井, 恵吏
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アライ, エリ
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Arai, Eri
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慶應義塾大学・医学部・講師
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Research team head
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科研費研究者番号 : 40446547
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後藤, 政広
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ゴトウ, マサヒロ
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Goto, Masahiro
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国立研究開発法人国立がん研究センター研究所・主任研究員
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Research team member
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科研費研究者番号 : 00291138
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2016
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科学研究費補助金研究成果報告書
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2015
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| Abstract |
肝部分切除術検体から, 計31検体分の正常肝細胞ならびに非がん肝細胞を純化し得た。比較対照の組織検体と共にゲノム網羅的DNAメチル化解析を行った。HCV陽性症例はHBV陽性症例に比してDNAメチル化異常の頻度が高く, HCV陽性症例ではDNAメチル化亢進の頻度が高かった。HBV特異的プロファイルは極めて少なく, 一方でHCV特異的プロファイルはゲノム全体に高頻度で認められた。組織検体と純化肝細胞の結果は概ね一致していたが, 純化細胞でより顕著に観察できる特徴もあった。純化細胞を用いることでChIP-seqが可能になり, 臨床情報と紐付いたヒト臨床検体のヒストン修飾パターン取得が可能になった。
Thirty-one samples of hepatocytes with precancerous and normal condition could be purified from partial hepatectomy specimens of patients with metastatic carcinoma and hepatocellular carcinoma associated with hepatitis B or C viral infection. Methylome analysis was performed using Infinium HumanMethylation450k BeadChip. The incidence of DNA methylation alterations was higher at the precancerous stage of HCV-positive patients than that of HBV-positive patients. Many Infinium probes showed HCV-positive-specific DNA methylation alterations throughout the genome, by contrast to HBV-positive patient-specific ones. Although DNA methylation profile of purified hepatocytes exhibited almost similar trend with liver tissue samples, some characteristic profiles appeared significant in purified cells. Cell purification from surgical specimen enabled histone modification profiling which is applicable to personal differentiation or clinical analysis.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2013～2015
課題番号 : 25460487
研究分野 : 病理学, エピゲノム
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| Jul 30, 2019 | | 著者,著者版フラグ を変更 |
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