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KAKEN_17H06563seika.pdf
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Download
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:93.3 KB
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:May 12, 2020 |
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Total downloads since May 12, 2020 : 284
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| Title |
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大脳皮質形成における環境ストレス応答下流カスケードの解析
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ダイノウ ヒシツ ケイセイ ニ オケル カンキョウ ストレス オウトウ カリュウ カスケード ノ カイセキ
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Dainō hishitsu keisei ni okeru kankyō sutoresu ōtō karyū kasukēdo no kaiseki
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Analysis of environmental stress response downstream cascade in cerebral cortex formation
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石井, 聖二
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イシイ, セイジ
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Ishii, Seiji
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慶應義塾大学・医学部 (信濃町)・特任講師
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Research team head
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科研費研究者番号 : 50468493
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2019
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科学研究費補助金研究成果報告書
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2018
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大脳皮質特異的に一次繊毛を欠損したマウス (Ift88 cKOマウス) を作製し、生後7日目のIft88 cKOマウスにアルコールを投与したところ、Ift88 cKOマウスでは、細胞質内に特異的な斑点状の活性型 Caspase-3シグナルが多数散在する事を見出した。そこで、アルコール曝露下でのcKOマウス群における神経細胞の樹状突起の長さ、および分枝の複雑度を解析したところ、いずれも優位に減少することを見出した。以上の結果から、臨界期後に一次繊毛に依存して誘導されるストレス応答シグナルの存在が示唆された。
To test the roles of cilia in brain development under exposure to environmental insults, we utilized a conditional knockout (cKO) of intraflagellar transport 88 (Ift88). Ift88 floxed mice were crossed with mice carrying the transgene Emx1-IRES-Cre (Emx1-Cre) by which Cre is expressed specifically in the dorsal pallium-originated cortical cells. The number of activated caspase-3-positive neurons remained small 24 hours post exposure in conditional heterozygote (cHet) control mice, while the number was significantly larger in cKO mice. We next found that dendritic complexity was significantly decreased; Thy1-YFP-labeled layer V neurons in EtOH-exposed cKO mice exhibited fewer dendrites with fewer and shorter branches than those in other groups. These results suggest that there might be the primary cilia-dependent neuroprotective function activated by environmental stress.
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研究種目 : 研究活動スタート支援
研究期間 : 2017～2018
課題番号 : 17H06563
研究分野 : 分子神経生物学
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| Oct 31, 2019 | | インデックス を変更 |
| May 12, 2020 | | Creator,Creator Affiliation,Abstract 内容,Note 注記 を変更 |
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