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KAKEN_17H03632seika.pdf
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:Mar 5, 2021 |
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Total downloads since Mar 5, 2021 : 219
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| Title |
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piRNA生合成経路の解析
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piRNA セイゴウセイ ケイロ ノ カイセキ
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piRNA seigōsei keiro no kaiseki
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Analysis of piRNA biogenesis pathway
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石津, 大嗣
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イシズ, ヒロツグ
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Ishizu, Hirotsugu
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慶應義塾大学・医学部 (信濃町) ・講師
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Research team head
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科研費研究者番号 : 40574588
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2020
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科学研究費補助金研究成果報告書
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2019
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piRNAは、相補的な配列を持つ遺伝子の発現を抑制する生殖細胞特異的な機能性小分子RNAである。申請者は、ショウジョウバエを用いたpiRNA生合成因子探索の結果、ATP依存性RNAヘリカーゼであるArmitage（Armi）を同定した。本研究により、細胞内でArmiに結合するRNAをiCLIP法および独自に開発したCLIPPARE法により解析することで、これまで詳細な解析がされていなかったpiRNA生合成遷移過程を捉えることが可能となった。また、生化学的解析により、piRNA生合成経路の詳細な分子メカニズムを解明した。
piRNAs are germ cell-specific functional small molecule RNAs that repress the expression of genes with complementary sequences. We identified an ATP-dependent RNA helicase, Armitage (Armi), as a piRNA biogenesis factor in Drosophila. By analyzing the RNA that binds to Armi in cells using iCLIP and CLIPPARE that we independently developed, we were able to capture the transition process of piRNA biogenesis, which has not been analyzed in detail. Biochemical analysis revealed a detailed molecular mechanism of the piRNA biogenesis pathway.
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研究種目 : 基盤研究 (B) (一般)
研究期間 : 2017～2019
課題番号 : 17H03632
研究分野 : 分子生物学
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