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KAKEN_17H03592seika.pdf
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Download
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:524.1 KB
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:Nov 14, 2022 |
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Total downloads since Nov 14, 2022 : 422
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オルガノイド培養による胆道・膵臓腫瘍細胞バンクの構築と個別化治療への応用
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オルガノイド バイヨウ ニ ヨル タンドウ・スイゾウ シュヨウ サイボウ バンク ノ コウチク ト コベツカ チリョウ エノ オウヨウ
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Oruganoido baiyō ni yoru tandō suizō shuyō saibō banku no kōchiku to kobetsuka chiryō eno ōyō
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Development of biliary and pancreatic tumor cell bank using organoid culture and its application to personalized therapy
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齋藤, 義正
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サイトウ, ヨシマサ
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Saitō, Yoshimasa
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慶應義塾大学・薬学部 (芝共立) ・准教授
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Research team head
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科研費研究者番号 : 90360114
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佐藤, 俊朗
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サトウ, トシロウ
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Satō, Toshirō
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慶應義塾大学・医学部・教授
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Research team member
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科研費研究者番号 : 70365245
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金井, 弥栄
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カナイ, ヤエ
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Kanai, Yae
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慶應義塾大学・医学部・教授
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Research team member
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科研費研究者番号 : 00260315
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金澤, 秀子
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カナザワ, ヒデコ
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Kanazawa, Hideko
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慶應義塾大学・薬学部・教授
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Research team member
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科研費研究者番号 : 10240996
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2020
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科学研究費補助金研究成果報告書
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2019
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本研究では、複数の胆道・膵臓がんの患者より提供されたがん組織を用いてオルガノイドを樹立し、1年以上にわたり安定的に培養・維持することに成功した。
これらの胆道・膵臓がんオルガノイドを用いて、遺伝子発現や遺伝子変異などを解析し、SOX2、KLK6、CPB2遺伝子の発現が、胆道・膵臓がん患者の予後を予測する新たなバイオマーカーとなる可能性を見出した。
さらに、既存薬ライブラリーを用いたスクリーニングを行ったところ、アモロルフィンやフェンチコナゾールなどの抗真菌薬がヒット化合物の中に含まれており、これらの薬剤が胆道がんや膵臓がんを効率的に抑制する新たな予防・治療薬の候補になると考えられた。
We successfully established organoids derived from biliary tract carcinoma (BTC) patients. These BTC organoids were cultured stably for over one year and closely recapitulated the histopathology, gene expression, and genetic alterations evident in the primary tumors. Gene expression profiling of the organoids and clinical data of patients revealed that SOX2, KLK6, and CPB2 could be a potential prognostic biomarker for patients with BTC. Moreover, we performed a drug screening with a compound library consisting of drugs employed clinically for their ability to suppress BTC organoids as a "drug repositioning" strategy. We discovered that the antifungal drugs amorolfine and fenticonalzole significantly suppressed the growth of BTC organoids. Antifungal drugs including amorolfine and fenticonalzole could be potentially applied for the prevention and treatment of BTC patients.
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研究種目 : 基盤研究 (B) (一般)
研究期間 : 2017～2019
課題番号 : 17H03592
研究分野 : 腫瘍治療学
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| Mar 5, 2021 | | インデックス を変更 |
| Nov 14, 2022 | | Creator Name,Creator Kana,Creator Romanization,Creator Affiliation,Creator Affiliation (Translated),Creator Role,Creator Link,Abstract 内容,Note 注記 を変更 |
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