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KAKEN_17H01433seika.pdf
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Download
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:316.8 KB
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:Nov 14, 2022 |
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Total downloads since Nov 14, 2022 : 194
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組織幹細胞の維持・若返りを可能にする新規分子メカニズム
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ソシキ カンサイボウ ノ イジ・ワカガエリ オ カノウ ニ スル シンキ ブンシ メカニズム
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Soshiki kansaibō no iji wakagaeri o kanō ni suru shinki bunshi mekanizumu
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Novel molecular mechanisms for enabling the maintenance and rejuvenation of tissue stem cells
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洪, 実
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コウ, ミノル
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Kō, Minoru
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慶應義塾大学・医学部 (信濃町) ・教授
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Research team head
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科研費研究者番号 : 50631199
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中武, 悠樹
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ナカタケ, ユウキ
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Nakatake, Yūki
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慶應義塾大学・医学部 (信濃町) ・講師
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Research team member
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科研費研究者番号 : 20415251
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秋山, 智彦
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アキヤマ, トモヒコ
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Akiyama, Tomohiko
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慶應義塾大学・医学部 (信濃町) ・助教
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Research team member
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科研費研究者番号 : 20570691
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洪, 繁
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コウ, シゲル
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Kō, Shigeru
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慶應義塾大学・医学部 (信濃町) ・准教授
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Research team member
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科研費研究者番号 : 90402578
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小田, 真由美
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オダ, マユミ
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Oda, Mayumi
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慶應義塾大学・医学部 (信濃町) ・特任講師
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Research team member
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科研費研究者番号 : 80567511
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2020
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科学研究費補助金研究成果報告書
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2019
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マウス成体組織におけるZscan4の発現解析および遺伝子改変マウスの作製を行った。(1) 成体マウス組織の各Zscan4パラログの発現解析を行い、精巣・卵巣・膵以外においても組織特異的に発現するパラログが確認された。免疫染色で一部の細胞にのみ陽性が認められＥＳ細胞と同様であった。(2) Zscan4遺伝子クラスター(850kb)領域をKOしたマウスを作成した。LoxP配列を挿入したconditional KO ES細胞からマウスを作成した。Zscan4 全クラスター領域が欠損したマウスも作成したが、germ lineへ入らなかった。今後、新たなconditional KOマウスの作成を進める。
Expression analysis of Zscan4 in adult mouse tissues and generation of genetically modified mice were performed. (1) An expression analysis of each Zscan4 paralog in adult mouse tissues was performed, and a paralog that was tissue-specifically expressed in areas other than the testis, ovary, and pancreas was confirmed. By immunostaining, only some cells were positive, which was similar to ES cells. (2) A mouse in which the Zscan4 gene cluster (850 kb) region was KO was prepared. Mice were prepared from conditional KO ES cells in which LoxP sequences were inserted. Mice deficient in the entire Zscan4 cluster region were also created, but did not enter the germ line. We will continue to develop new conditional KO mice.
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研究種目 : 基盤研究 (A) (一般)
研究期間 : 2017～2019
課題番号 : 17H01433
研究分野 : システム医学
PDFファイルは改訂版に差し替え (2022.11.11)
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| Mar 5, 2021 | | インデックス を変更 |
| Nov 11, 2022 | | Preview,Full text,Creator Name,Creator Kana,Creator Romanization,Creator Affiliation,Creator Affiliation (Translated),Creator Role,Creator Link,Creator 著者ID,Abstract 内容,Note 注記 を変更 |
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