| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_15K14431seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:874.6 KB
|
| Last updated |
:Sep 21, 2017 |
| Downloads |
: 489 |
Total downloads since Sep 21, 2017 : 489
|
|
| Release Date |
|
| Title |
| Title |
Gene body領域エピゲノムのモニタリングによる細胞成熟誘導法の最適化
|
| Kana |
Gene body リョウイキ エピゲノム ノ モニタリング ニ ヨル サイボウ セイジュク ユウドウホウ ノ サイテキカ
|
| Romanization |
Gene body ryoiki epigenomu no monitaringu ni yoru saibo seijuku yudoho no saitekika
|
|
| Other Title |
| Title |
The establishment of cell maturation induction method by the gene body-centered epigenomic monitoring.
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
小田, 真由美
 |
| Kana |
オダ, マユミ
|
| Romanization |
Oda, Mayumi
|
| Affiliation |
慶應義塾大学・医学部・助教
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 80567511
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2017
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2016
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
本研究計画はglobal DNAメチル化修飾をバックグラウンドとする哺乳類ゲノムにおいて, gene body領域のエピジェネティック状態が細胞成熟に関与する可能性を検討し, これをもとに細胞成熟の評価および誘導を目指す。当初の成熟誘導法の樹立の計画に先立ち心筋細胞と肝細胞の比較を行ったところ, 低メチル化を特徴とするgene body領域エピジェネティック・ドメインは心筋細胞により強く, 他の細胞にはあまり顕著に見られない特徴であることがわかった。また, DNA脱メチル化代謝中間産物の解析により酸化的脱メチル化過程がこの活性エピジェネティック・ドメインの構築に関与している可能性が示された。
This research project aims to evaluate and induce cell maturation on the basis of the possibility that the epigenetic state of the gene body region may be involved in cell maturation in a mammalian genome whose background is global DNA methylation modification. Comparison of cardiomyocytes and hepatocytes prior to the establishment of the initial maturation induction method revealed that the gene body epigenetic domain characterized by DNA hypomethylation is evident in cardiomyocytes and less pronounced in other cell types. Analysis of DNA demethylation metabolic intermediates in this cardiomyocyte-specific epigenetic domain also showed that the oxidative demethylation process might be involved in the construction of this active epigenetic domains.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 挑戦的萌芽研究
研究期間 : 2015～2016
課題番号 : 15K14431
研究分野 : エピゲノム学
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
|
| Index |
|
| Related to |
|
