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AN10079809-20210930-0023.pdf
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Download
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:3.4 MB
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| Last updated |
:Sep 30, 2021 |
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: 195 |
Total downloads since Sep 30, 2021 : 195
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| Title |
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Biotin-streptavidin linkage utilized to fabricate dense packing of red blood cells immobilized to protein monolayer on solid support
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ビオチン-ストレプトアビジン結合を利用して赤血球の稠密パッキングを固体基板表面のタンパク質単分子膜に固定化する方法
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| Kana |
ビオチン-ストレプトアビジン ケツゴウ オ リヨウシテ セッケッキュウ ノ チュウミツ パッキング オ コタイ キバン ヒョウメン ノ タンパクシツ タンブンシマク ニ コテイカスル ホウホウ
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| Romanization |
Biochin-sutoreputoabijin ketsugō o riyōshite sekkekkyū no chūmitsu pakkingu o kotai kiban hyōmen no tanpakushitsu tanbunshimaku ni koteikasuru hōhō
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古野, 泰二
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フルノ, タイジ
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Furuno, Taiji
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| Affiliation |
慶應義塾大学日吉物理学教室講師 (非常勤); 慶應義塾大学名誉教授
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Part-time lecturer, Department of Physics, Hiyoshi Campus, Keio University; Professor emeritus, Keio University.
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慶應義塾大学日吉紀要刊行委員会
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ケイオウ ギジュク ダイガク ヒヨシ キヨウ カンコウ イインカイ
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Keiō gijuku daigaku Hiyoshi kiyō kankō iinkai
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2021
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慶應義塾大学日吉紀要. 自然科学
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The Hiyoshi review of natural science
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68
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2021
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9
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| Start page |
23
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32
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| Abstract |
Immobilization of red blood cells (RBCs) onto the surface of solid support is described. Biotinylated carbonic anhydrase (CA) was spread at the air/water interface, unfolded there, transferred onto silicon wafer, and coupled with streptavidin (SAv). Biotinylated RBCs were bound to this SAv layer via biotin-streptavidin linkage forming an immobilized dense packing of RBCs. Upon hemolysis the dense packing of disk-shaped RBCs changed to an in-plane packing of polygon-shaped flattened cells contacting with each other without void space. As an application, patterning of the RBC dense packing was examined.
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| Keyword |
| Langmuir-Schaefer technique
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| Departmental Bulletin Paper
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