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KAKEN_26462537seika.pdf
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Download
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| Title |
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新規網羅的スクリーニング法による卵巣癌薬剤耐性遺伝子の検索
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シンキ モウラテキ スクリーニングホウ ニ ヨル ランソウガン ヤクザイ タイセイ イデンシ ノ ケンサク
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Shinki mōrateki sukurīninguhō ni yoru ransōgan yakuzai taisei idenshi no kensaku
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Search for drug resistance genes in ovarian cancer cells by new exhaustive genome screening method
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野村, 弘行
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ノムラ, ヒロユキ
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Nomura, Hiroyuki
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慶應義塾大学・医学部(信濃町)・助教
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Research team head
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科研費研究者番号 : 50327590
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片岡, 史夫
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カタオカ, フミオ
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Kataoka, Fumio
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Research team member
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山上, 亘
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ヤマガミ, ワタル
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Yamagami, Wataru
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Research team member
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千代田, 達幸
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チヨダ, タツユキ
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Chiyoda, Tatsuyuki
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Research team member
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井口, 蓉子
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イグチ, ヨウコ
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Iguchi, Yoko
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橋本, 志歩
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ハシモト, シホ
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Hashimoto, Shiho
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岩佐, 尚美
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イワサ, ナオミ
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Iwasa, Naomi
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Research team member
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中平, 直希
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ナカダイラ, ナオキ
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Nakadaira, Naoki
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吉浜, 智子
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ヨシハマ, トモコ
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Yoshihama, Tomoko
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南木, 佳子
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ナンキ, ヨシコ
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Nanki, Yoshiko
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Research team member
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同前, 愛
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ドウゼン, アイ
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Dozen, Ai
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早乙女, 啓子
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サオトメ, ケイコ
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Saotome, Keiko
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田中, 英雄
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タナカ, ヒデオ
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Tanaka, Hideo
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Research team member
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2018
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科学研究費補助金研究成果報告書
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2017
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| Abstract |
本研究では, 薬剤耐性遺伝子をゲノムワイドで同定可能な新規遺伝子スクリーニング法を用いて, 卵巣癌細胞株における薬剤耐性遺伝子の同定を試みることを目的とした。また, ストレス応答・薬剤耐性に関与する遺伝子マーカーと一塩基多型(SNP)との関連性の検証を行った。
薬剤耐性遺伝子スクリーニングのための卵巣癌細胞株の薬剤耐性コロニーの作成と基礎的なデータを得ることができ, 実験系の条件設定の検証を行うことができた。しかしながら, 一部の組織型由来の細胞株ではコロニーの安定した樹立が困難であり, 本手法の適用に課題を残した。また, タキサン代謝関連遺伝子上のSNPと抗がん剤感受性との相関が示唆された。
We aimed to identify drug resistance gene in ovarian cancer cell line using a novel wide gene screening method. We also examined the association between single nucleotide polymorphisms (SNPs) and genetic markers involved in stress response or drug resistance.
We were able to obtain drug-resistant colonies of ovarian cancer cell lines for screening drug resistant genes and obtain basic data, and to verify experimental condition setting. However, it was difficult to stably establish colonies in cell lines derived from some histological types, leaving a problem in applying this method widely. In addition, a correlation between SNPs on taxane metabolism-related genes and sensitivity to cytotoxic drugs was suggested.
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研究種目 : 基盤研究(C)(一般)
研究期間 : 2014～2017
課題番号 : 26462537
研究分野 : 産婦人科学
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| History |
| Nov 12, 2018 | | インデックス を変更 |
| Jul 30, 2019 | | 出版地 場所,出版者 名前,出版者 カナ,出版者 ローマ字,日付 出版年(from:yyyy),日付 出版年(to:yyyy),日付 作成日(yyyy-mm-dd),日付 記録日(yyyy-mm-dd),形態,上位タイトル 名前,上位タイトル 翻訳,上位タイトル 巻,上位タイトル 号,上位タイトル 年,上位タイトル 月,上位タイトル 開始ページ,上位タイトル 終了ページ,ISSN,ISBN,DOI,URI,JaLCDOI,NII論文ID,医中誌ID,その他ID,博士論文情報 学位授与番号,博士論文情報 学位授与年月日,博士論文情報 学位名,博士論文情報 学位授与機関,抄録 内容,目次,キーワード,NDC,注記,言語,資源タイプ,ジャンル,著者版フラグ,本文URI URI,アクセス条件 を変更 |
| Jul 30, 2019 | | 著者 を変更 |
| Apr 22, 2024 | | Creator を変更 |
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