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KAKEN_25560406seika.pdf
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| Title |
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卵殻と貝殻のカルシウム移動を司る有機分子の探索
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| Kana |
ランカク ト カイガラ ノ カルシウム イドウ オ ツカサドル ユウキ ブンシ ノ タンサク
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Rankaku to kaigara no karushiumu ido o tsukasadoru yuki bunshi no tansaku
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Investigation of organic molecules responsible for calcium translocation in eggshell and nacre shell
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犀川, 陽子
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サイカワ, ヨウコ
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Saikawa, Yoko
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| Affiliation |
慶應義塾大学・理工学部・准教授
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Research team head
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科研費研究者番号 : 20348824
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末松, 誠
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スエマツ, マコト
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Suematsu, Makoto
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慶應義塾大学医学部・教授
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Research team member
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久保, 亜紀子
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クボ, アキコ
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Kubo, Akiko
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慶應義塾大学医学部・講師
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Research team member
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今井, 祐記
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イマイ, ユウキ
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Imai, Yuki
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| Affiliation |
愛媛大学医学部・教授
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Research team member
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2016
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科学研究費補助金研究成果報告書
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2015
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| Abstract |
鳥類の卵殻から胚へのカルシウム移動に寄与する有機分子の探索を行った。ダチョウの卵殻から一種のリン酸エステルを含む4種の成分を同定し, その中のリン酸エステルAは結晶よりも溶解性の良い非晶質の炭酸カルシウムの生成を誘起することを明らかにした。また, 卵殻成分の定量およびマスイメージングを行い, Aは骨形成の際に溶解する卵殻内側部分に局在することがわかった。よって, Aは卵殻からのスムーズなカルシウムイオンの溶解と移動に寄与していると言える。一方, 南洋真珠の貝殻が骨芽細胞の分化を活性化する現象に注目した。検討により, 骨芽細胞の活性化を再現するためには, 貝殻小片が培養系に共存する必要があることがわかった。
We investigated organic compounds which contributes to calcium translocation from eggshell to chick embryo. From the ostrich eggshell, we identified four compounds including an organophosphate. Among these compounds, the organophosphate A turned out to induce the formation of amorphous calcium carbonate. In addition, quantification of eggshell components by stepwise extraction revealed that organophosphate A was located in the inner layer of eggshell and decreased as an embryo bone is formed. This localization was also proved by MALDI-TOF imaging analysis of cross section of eggshell. The above results support the assumption that organophosphate A assists decalcification and translocation of calcium ion. On the other hand, we focused on the shell of nacre (pinctada maxima) which is said to promote the differentiation of osteoblast. We found that the reproducible differentiation and calcification of the osteoblast require not the extract of nacre powder, but nacre chips.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2013～2015
課題番号 : 25560406
研究分野 : 天然物化学, 有機合成化学
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