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KAKEN_24791721seika.pdf
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Title |
Title |
ヒト卵管幹細胞の同定と解析
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Kana |
ヒト ランカン カンサイボウ ノ ドウテイ ト カイセキ
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Hito rankan kansaibo no dotei to kaiseki
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Identification and characterization of stem/progenitor cells in the human fallopian tube
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内田, 明花
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Kana |
ウチダ, サヤカ
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Uchida, Sayaka
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慶應義塾大学・医学部・共同研究員
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Research team head
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科研費研究者番号 : 60445236
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2015
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科学研究費補助金研究成果報告書
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2014
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Abstract |
卵管幹細胞の同定に必要なin vivo卵管組織再構成システムの確立に際して, その基盤となる技術開発を不死化内膜間質細胞と不死化内膜腺上皮細胞を用いて行った。まず, これらの細胞株を出発材料として, 発光・蛍光蛋白を安定的に発現する細胞株を作成した。さらに, 標識細胞が組織再構成の過程のなかで, どのように振る舞うかを検証する細胞追跡システムを開発した。本研究により, 不死化卵管上皮細胞株が利用可能になった際, その再構成システムと幹細胞追跡法への応用が可能な基盤知見が得られた。
In vivo reconstitution system of human fallopian tube (hFT) is needed to identify and characterize stem cells of hFT in vivo. Using immortalized human endometrial cells, we here attempted to obtain fundamental knowledge and techniques to realize the hFT reconstitution and cell tracking system. For this purpose, we first made endometrial cell lines stably expressing bioluminescent and fluorescent proteins. We then developed a method to introduce genes of interest into cells without cell culture and established a cell tracking system through the combinatory use of our labeling method and a tissue reconstitution system. The results of this study will be useful for identification and characterization of putative hFT stem cells when a hFT cell line becomes available.
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研究種目 : 若手研究(B)
研究期間 : 2012~2014
課題番号 : 24791721
研究分野 : 産婦人科, 生殖医学, 臨床遺伝学, 生殖内分泌学
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Jul 26, 2019 | | 著者,抄録 内容,ジャンル を変更 |
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