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KAKEN_22681029seika.pdf
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:673.3 KB
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:Dec 11, 2014 |
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| Title |
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マルチオミクス解析によるクマムシの乾燥耐性・極限環境耐性分子機構の解明
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マルチ オミクス カイセキ ニ ヨル クマムシ ノ カンソウ タイセイ・キョクゲン カンキョウ タイセイ ブンシ キコウ ノ カイメイ
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Maruchi omikusu kaiseki ni yoru kumamushi no kanso taisei kyokugen kankyo taisei bunshi kiko no kaimei
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Multi-omics study to elucidate the molecular mechanism of anhydrobiosis in tardigrade Ramazzottius varieornatus
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荒川, 和晴
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アラカワ, カズハル
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Arakawa, Kazuharu
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慶應義塾大学大学院・政策・メディア研究科・特任講師
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Research team head
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科研費研究者番号 : 40453550
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2014
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科学研究費補助金研究成果報告書
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2013
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陸生のクマムシの多くは周囲の環境の乾燥により、ほぼ完全な脱水を伴って生命活動を可逆的停止する「乾眠」という機構を持つが、その分子機構は未だ明らかではない。そこで、本研究ではヨコヅナクマムシ乾眠前後の主にメタボロームとリン酸化プロテオームによるマルチオミクスにより、有意に変動を示す鍵分子を特定すると共に、その制御機構を解析した。結果、CE-TOFMSとLC-TOFMSを組み合わせたメタボロミクスにより、主に酸化ストレス応答や抗浸透圧ストレス関連の分子の変動がみられ、それらを合成・分解する経路の多くが乾眠時にリン酸化されていることを明らかにした。
Limno-terrestrial tardigrades can withstand extreme environments and almost complete desiccation through a mechanism called anhydrobiosis, but the its molecular mechanism is yet to be explored. To this end, we have conducted a comprehensive multi-omics analysis using the tardigrade Ramazzottius varieornatus, which is a potential model species for anhydrobiosis. In order to analyze the metabolic changes in the active and dehydrated states, we measured the metabolome in both conditions using two types of high-throughput mass spectrometry (MS) systems, CE-TOFMS and LC-TOFMS. Moreover, phospho-proteome was also analyzed to observed the metabolic regulations. As a result, while changes in gene expression profiles are limited in between active and tun states, dynamic changes were observed in the metabolism of this species in response to desiccation. Changes in the metabolic profiles suggests complex intracellular responses to oxidative and osmotic stress.
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研究種目 : 若手研究(A)
研究期間 : 2010～2013
課題番号 : 22681029
研究分野 : 複合新領域
科研費の分科・細目 : ゲノム科学・ゲノム生物学
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