炎症性腸疾患発症に関して特異的な腸内細菌への絞込みを目的とし, 健常人(HC), 原発性硬化性胆管炎合併潰瘍性大腸炎(PSC/UC)患者糞便を無菌マウスへ移植したマウス(HCマウス, PSC/UCマウス)を作成し, PSC/UCマウスでは肝臓内Th17細胞の集積を認めた。PSC/UCマウスのみでKlebsiella pneumonia, Proteus mirabilis, Enterococcus gallinarumの同定培養に成功。Q-PCR法にて解析しPSC/UC患者に高頻度にこれらの腸内細菌が検出。ある特定の悪玉菌の腸内細菌が腸管外病変へ関与する事を見い出し, 発展性が期待できる成果と考える。
Regarding the onset of inflammatory bowel disease, it is important to reduce the diversity of intestinal microbiota. We generated the mice inoculated with healthy human feces (HC mice) or primary sclerosis cholangitis-associated ulcerative colitis (PSC/UC) patient feces (PSC/UC mice) and analysed a week after transplantation. In PSC/UC mice, Th17 cells was accumulated in liver compare to the HC mice. Next, we isolated 3 dominant microbiota in mesenteric lymph node of PSC/UC mice. Klebsiella pneumonia, Proteus mirabilis and Enterococcus gallinarum were isolated.We also analysed fecal microbiota in Healthy controls and PSC/UC patients. Analysis by the Q-PCR method confirmed that these intestinal bacteria were detected more frequently in PSC/UC patients than in HC control. We demonstrated that certain intestinal microbiota are involved in extraintestinal lesions. We will further promote vaccine therapy with the analysis of these bacteria.