The purpose of this study was to examine the effects of physical activity in P. E. class (aerobic dance exercise) on not only the physical but also on the psychological health of college-aged females. The healthy female students participated in the class once a week for a year and/or half a year. Changes in health-related physical fitness (HRPF) test scores and mental health check scores were compared between the pre-and post-P.E. class periods. The results demonstrated no significant changes in body composition, cardiovascular endurance capacity, flexibility, and muscular power, all of which were indices of HRPF. Only muscular endurance increased significantly during the period. Regular exercise once a week in a P.E. class is unlikely to remarkably improve an individual's physical fitness. On the other hand, an effect was revealed in the relationship between regular exercises on reduction of mental stress. And it was also demonstrated that the nutritional problems, which involved deficiency of iron, calcium, dietary fiber, and carbohydrate from the survey of their dietary life. These results show the importance of considering the health of collegiate females in terms of a combination of factors, including their physical fitness, psychological well-being, nutrition, and soon.