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jkup2007_2_001.pdf
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Title |
Title |
Quantitative expression of AMPA receptor subunits in HEK293 cells
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Kana |
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Creator |
Name |
鈴木, 岳之
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Kana |
スズキ, タケシ
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Romanization |
Suzuki, Takeshi
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Affiliation |
Department of Basic Biological Sciences, Kyoritsu University of Pharmacy
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下遠野, 久美子
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Kana |
シモトオノ, クミコ
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Romanization |
Shimotohno, Kumiko
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Affiliation |
Department of Basic Biological Sciences, Kyoritsu University of Pharmacy
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Nakamura, Takeshi
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Department of Physiology, Juntendo University School of Medicine
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Brorson, James R.
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Department of Neurology, University of Chicago
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Name |
共立薬科大学
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Kana |
キョウリツ ヤッカ ダイガク
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Romanization |
Kyoritsu yakka daigaku
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Date |
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2007
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Physical description |
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Source Title |
Name |
共立薬科大学雑誌
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The journal of Kyoritsu University of Pharmacy
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Volume |
2
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Year |
2007
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3
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1
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8
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Abstract |
The participation of AMPA receptors in certain neurodegenerative diseases may depend on their Ca2+ permeability. AMPA receptors, which consist of multimetric assemblies of four (or five) subunits, can be divided into two subclasses, those with low (type I) and high Ca2+ permeability (type II). We transfected cDNAs of GluR1 and GluR2 AMPA receptor subunits in varying ratios into HEK293 cells to clarify the subunit ratio-dependence of glutamate responses. An implicit assumption in this study is that levels of protein subunit expression reliably reflect cDNA transfection ratios. Analysis of whole-cell currents generated from co-expression of GluR1 and GluR2 indicated that two functionally distinct receptors are produced (types I and II). When GluR1 and GluR2 were co-transfected in a 4:1 ratio, the resulting whole-cell current traces exhibited properties that were a mixture of those of type I and type II receptors. The experimental system of quantitative subunit protein expression developed in this study is useful for electrophysiological study of AMPA receptor functions.
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Departmental Bulletin Paper
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