ラット心停止モデルを用い, 生存率や行動実験, 脳組織を検討した。水素吸入群では, 脳機能スコアおよび生存率が対照と比較して著しく改善した。水素吸入は顕著な改善効果を認めた。対照では脳海馬における神経細胞死数, 軸索損傷, ミクログリア, および大脳皮質の神経細胞変性やアストロサイトの変化が認められたが, 水素群ではそれらが抑制された。ラット出血性ショックモデルにおいても, 対照では肺組織のびまん肺胞損傷, 肺胞内好中球, 赤血球, 細胞死組織片, 硝子膜形成を伴う上皮基底膜好中球の浸潤をみとめたが水素群ではこれらが軽減していた。一方, マイクロパーティクルは一定したシグナル検出が得られず, 今後の検討が必要である。
Rats were subjected to 6 minutes of cardiac arrest followed by cardiopulmonary resuscitation. Survival rate after resuscitation was 38.4% in the control group, 71.4% in the H2 and targeted temperature management (TTM) groups, and 85.7% in the TTM+H2 group. Combined therapy of TTM and H2 inhalation was superior to TTM alone in terms of neurological deficit scores at 72 hours after resuscitation. Neuronal degeneration and microglial activation in a vulnerable brain region was suppressed by both TTM alone and H2 inhalation alone, with the combined therapy of TTM and H2 being most effective. In a hemorrhage shock model, the survival rate was improved in H2 group compared to control group. IL-6 level was increased in the control group compared with sham, but this increase was prevented in the H2 group. Lung of control group showed massive cellular infiltration and edema in the interstitial area and associated thickening of the alveolar septum. These changes were less severe in H2 gas group.
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