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KAKEN_26600055seika.pdf
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Download
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:492.0 KB
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:Jan 6, 2017 |
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Total downloads since Jan 6, 2017 : 744
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| Title |
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シリコンナノポアを用いた光学検出による一分子DNAの塩基配列情報解読
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シリコン ナノポア オ モチイタ コウガク ケンシュツ ニ ヨル イチブンシ DNA ノ エンキ ハイレツ ジョウホウ カイドク
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Shirikon nanopoa o mochiita kogaku kenshutsu ni yoru ichibunshi DNA no enki hairetsu joho kaidoku
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Optical detection of single DNA molecules using a silicon nanopore for readout of sequence information
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斎木, 敏治
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サイキ, トシハル
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Saiki, Toshiharu
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慶應義塾大学・理工学部・教授
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Research team head
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科研費研究者番号 : 70261196
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岩崎, 渉
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イワサキ, ワタル
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Iwasaki, Wataru
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東京大学・大学院理学系研究科・准教授
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Research team member
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科研費研究者番号 : 50545019
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2016
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科学研究費補助金研究成果報告書
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2015
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ナノポアシーケンサにおいて高精度な塩基識別実現に向けた課題は, 通過速度の低速化と安定化である。そのためには, ナノポア通過直前・直後のDNAの形態やそれに依存したドリフト運動と拡散運動の競合等を明らかにする必要がある。本研究では, ナノポア通過中, ならびに通過後のDNAコイル形成と空間的移動の観測に必要な100nm, 0.1msの空間・時間分解能を有する光学的観察手法の開発, 動作実証をおこない, さらにナノポア近傍の電場に起因する特徴的なDNA通過挙動を見出した。また塩基情報解読に向けてSTED利用の有効性を確認し, プラズモニック・ナノポアの作製に成功した。
The challenge in highly accurate identification of each nucleotide is slowing and stabilizing DNA translocation velocity. For this purpose, DNA conformation and drift-diffusion motion immediately before and after translocation should be investigated. In this study, an optical microscopic method with 100-nm and 0.1-ms resolution is developed to observe DNA coiling immediately after translocation and its motion. Characteristic DNA translocation dynamics caused by the electric field in the vicinity of nanopore exit is obtained and the mechanism is discussed in detail. To achieve higher spatial resolution aiming to single-molecule sequencing, the possibility of combination with STED and plasmonic nanopore is also demonstrated.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2014～2015
課題番号 : 26600055
研究分野 : ナノ光学
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