舌癌は頸部リンパ節転移が生じやすい癌である。後発頸部リンパ節転移と癌幹細胞との関連および, 血管内皮細胞増殖因子と頸部リンパ節転移について検討した。RT-PCRにて癌幹細胞関連遺伝子の発現を舌癌細胞株の癌幹細胞様細胞のSP細胞とnon SP細胞で比較した結果, SP細胞でOct3/4, Nanogの発現が高く, また遊走能と浸潤能が高かった。stage I/II舌癌検体に, Oct3/4とNanogの発現を免疫染色にて評価した。多変量解析ではOct3/4発現とVascular invasionが独立相関因子であった。Oct3/4は舌癌の有用な癌幹細胞マーカーと考えられた。
In the present study, we aimed to determine the CSC markers in the SP of HNSCC cells along with their functions in cellular behaviors, and to clarify the association of these markers with delayed neck metastasis (DNM). Results : SP were isolated in cell lines examined. Expression levels of Oct3/4 and Nanog were higher in SP cells than MP cells. Additionally, cell migration and invasion abilities were higher in SP cells than MP cells, whereas there was no difference in proliferation. Univariate analysis showed that Expression of Oct3/4 and Nanog, vascular and muscular invasion, and mode of invasion were significantly correlated with DNM. Multivariate logistic regression revealed that Oct3/4 expression and vascular invasion were independently predictive of DNM.Conclusion : These results suggest that Oct3/4 and Nanog represent probable CSC markers in HNSCC, which contribute to the development of DNM in part by enhancing cell motility and invasiveness.
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