| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_25430093seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:603.2 KB
|
| Last updated |
:Jul 26, 2019 |
| Downloads |
: 1149 |
Total downloads since Jul 26, 2019 : 1149
|
|
| Release Date |
|
| Title |
| Title |
実験用途に応じたNASH肝癌メダカ・マウス・ブタモデルの作成と予防・治療法の開発
|
| Kana |
ジッケン ヨウト ニ オウジタ NASH カンガン メダカ・マウス・ブタ モデル ノ サクセイ ト ヨボウ・チリョウホウ ノ カイハツ
|
| Romanization |
Jikken yoto ni ojita NASH kangan medaka mausu buta moderu no sakusei to yobo chiryoho no kaihatsu
|
|
| Other Title |
| Title |
Development of a novel animal model of hepatocellular carcinoma with nonalcoholic steatohepatitis
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
相浦, 浩一
|
| Kana |
アイウラ, コウイチ
|
| Romanization |
Aiura, Koichi
|
| Affiliation |
慶應義塾大学・医学部・講師(非常勤)
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 00184010
|
| Name |
板野, 理
|
| Kana |
イタノ, オサム
|
| Romanization |
Itano, Osamu
|
| Affiliation |
慶應義塾大学・医学部・講師
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 90265827
|
| Name |
北郷, 実
 |
| Kana |
キタゴウ, ミノル
|
| Romanization |
Kitagō, Minoru
|
| Affiliation |
慶應義塾大学・医学部・講師
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 70296599
|
| Name |
松田, 祐子
|
| Kana |
マツダ, サチコ
|
| Romanization |
Matsuda, Sachiko
|
| Affiliation |
慶應義塾大学・医学部・特任講師
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 90534537
|
| Name |
大西, 彰
|
| Kana |
オオニシ, アキラ
|
| Romanization |
Onishi, Akira
|
| Affiliation |
日本大学・生物資源科学部・教授
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 30414890
|
| Name |
小島, 美咲
|
| Kana |
コジマ, ミサキ
|
| Romanization |
Kojima, Misaki
|
| Affiliation |
独立行政法人農業生物資源研究所・家畜ゲノム研究ユニット・上級研究員
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 80355742
|
| Name |
谷口, 善仁
|
| Kana |
タニグチ, ヨシヒト
|
| Romanization |
Taniguchi, Yoshihito
|
| Affiliation |
杏林大学・医学部・教授
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 00324616
|
| Name |
吉浦, 康寿
|
| Kana |
ヨシウラ, ヤストシ
|
| Romanization |
Yoshiura, Yasutoshi
|
| Affiliation |
独立行政法人水産総合研究センター・増養殖研究所・研究員
|
| Affiliation (Translated) |
|
| Role |
Research team member
|
| Link |
科研費研究者番号 : 90372052
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2016
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2015
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
本研究では, 非アルコール性脂肪肝炎(NASH)による障害肝からの発癌動物モデルを新規作製した。3週齢のC57BLマウスにHFCD(低コリン脂肪食)を与えると共に, 開始時にper kg 25mgのDENを腹腔内投与した。HFCD群では12週以降でほぼ100%インスリン抵抗性を獲得した。HFCD＋DENは脂肪沈着の他に肝細胞の風船様変性マロリー体などの所見があり, よりNASHに近い組織像が観察され, 腫瘍形成も20週目から確認された。
The aim of this study was to develop a mouse model of hepatocellular carcinoma with nonalcoholic steatohepatitis that develops quickly and reflects all clinically relevant features.
Three-week-old C57BL/6J male mice were fed either a standard diet (MF) or a choline-deficient, high-fat diet (HFCD). The mice in the MF+diethylnitrosamine (DEN) and HFCD+DEN groups received a one-time intraperitoneal injection of DEN at the start of the respective feeding protocols. The mice in the HFCD and HFCD+DEN groups developed obesity early in the experiment and insulin resistance after 12 weeks. The HFCD+DEN group presented the highest incidence of nonalcoholic steatohepatitis. The levels of fibrosis and steatosis varied, but they tended to increase every 4 weeks in the HFCD and HFCD+DEN groups. Computed tomography scans indicated that all the HFCD+DEN mice developed hepatic tumors from 20 weeks, some of which were glutamine synthetase-positive.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 基盤研究(C)(一般)
研究期間 : 2013～2015
課題番号 : 25430093
研究分野 : 外科学
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
| Jul 26, 2019 | | 著者,上位タイトル 名前 を変更 |
|
|
| Index |
|
| Related to |
|
