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KAKEN_24791718seika.pdf
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Download
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:242.5 KB
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:Apr 11, 2016 |
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Total downloads since Apr 11, 2016 : 680
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子宮体癌におけるmiRNA治療薬の開発および診断への応用
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シキュウタイガン ニ オケル miRNA チリョウヤク ノ カイハツ オヨビ シンダン エノ オウヨウ
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Shikyutaigan ni okeru miRNA chiryoyaku no kaihatsu oyobi shindan eno oyo
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Application to the development and diagnosis of miRNA therapeutic agent in endometrial cancer
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矢野倉, 恵
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ヤノクラ, メグミ
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Yanokura, Megumi
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慶應義塾大学・医学部・特任助教
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Research team head
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科研費研究者番号 : 20433732
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2015
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科学研究費補助金研究成果報告書
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2014
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microRNAは, 癌の発症・進展・薬剤耐性とも大きく関わることが明らかになっている。我々は, 子宮体癌においてエピジェネティックに発現抑制される癌抑制型microRNAとしてmiR-34bを同定し, さらにmiR-34bと抗癌剤の併用による抗腫瘍効果を検討した。
3種の子宮体癌細胞株に対しパクリタキセルを作用させた時は, 全ての細胞株においてmiR-34b添加時に細胞生存率の低下が認められた。また, HEC-1Bをヌードマウスの皮下に移植後, 28日目にはパクリタキセル+miR-34b群は他の群に比し有意に腫瘍径の縮小が認められた(P<0.05)。
microRNAs have key roles in the onset, development and drug resistance of cancer. We identified miR-34b as a tumor suppressor-type microRNA with expression that is epigenetically suppressed in endometrial cancer. In this study, the antitumor effect of combined miR-34b and antitumor drugs on endometrial cancer was examined. [Results] There was no change in cell viability after administration of miR-34b following application of cisplatin or adriamycin to HEC-108, HEC-1B and KLE cells. However, after treatment with paclitaxel, cell viability after administration of miR-34b decreased in all three cell lines. Tumor development occurred 14 days after transplantation of HEC-1B cells in nude mice. Drugs were administered on that day and thereafter. The tumor diameter significantly decreased in mice treated with paclitaxel + miR-34b compared with the results for other treatments (P<0.05). The GFP fluorescence signal also markedly decreased after treatment with paclitaxel + miR-34b.
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研究種目 : 若手研究(B)
研究期間 : 2012～2014
課題番号 : 24791718
研究分野 : 婦人科腫瘍学
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