| Item Type |
Article |
| ID |
|
| Preview |
| Image |
|
| Caption |
|
|
| Full text |
KAKEN_24791470seika.pdf
| Type |
:application/pdf |
Download
|
| Size |
:154.2 KB
|
| Last updated |
:Dec 11, 2014 |
| Downloads |
: 772 |
Total downloads since Dec 11, 2014 : 772
|
|
| Release Date |
|
| Title |
| Title |
人工酸素運搬体投与による肺移植後拒絶反応軽減の基礎的研究
|
| Kana |
ジンコウ サンソ ウンパンタイ トウヨ ニ ヨル ハイ イショクゴ キョゼツ ハンノウ ケイゲン ノ キソテキ ケンキュウ
|
| Romanization |
Jinko sanso unpantai toyo ni yoru hai ishokugo kyozetsu hanno keigen no kisoteki kenkyu
|
|
| Other Title |
| Title |
Hemoglobin vesicles ameriolate allograft airway rejection
|
| Kana |
|
| Romanization |
|
|
| Creator |
| Name |
加勢田, 馨
 |
| Kana |
カセダ, カオル
|
| Romanization |
Kaseda, Kaoru
|
| Affiliation |
慶應義塾大学・医学部・助教
|
| Affiliation (Translated) |
|
| Role |
Research team head
|
| Link |
科研費研究者番号 : 70624435
|
|
| Edition |
|
| Place |
|
| Publisher |
|
| Date |
| Issued (from:yyyy) |
2014
|
| Issued (to:yyyy) |
|
| Created (yyyy-mm-dd) |
|
| Updated (yyyy-mm-dd) |
|
| Captured (yyyy-mm-dd) |
|
|
| Physical description |
|
| Source Title |
| Name |
科学研究費補助金研究成果報告書
|
| Name (Translated) |
|
| Volume |
|
| Issue |
|
| Year |
2013
|
| Month |
|
| Start page |
|
| End page |
|
|
| ISSN |
|
| ISBN |
|
| DOI |
|
| URI |
|
| JaLCDOI |
|
| NII Article ID |
|
| Ichushi ID |
|
| Other ID |
|
| Doctoral dissertation |
| Dissertation Number |
|
| Date of granted |
|
| Degree name |
|
| Degree grantor |
|
|
| Abstract |
BALB/cマウスから気管を摘出し臓器保存液中に4時間保存後、異種移植としてC57BL/6マウスに移植した。1週間後に犠牲死させた。また人工酸素運搬体を臓器保存液中に加えた中に4時間保存後異所移植としてC57BL/6マウスに移植し1週間後に犠牲死させた。Real time PCRで炎症性サイトカインの測定、また病理組織での拒絶反応を検討した。検討したマウスの数が少ないためか人工酸素運搬体投与群で拒絶反応が抑制された傾向はあったが有意差は認めなかった。今後はＴ細胞の表面抗原の発現によりどのＴ細胞が関与しているか検討する必要がある。
Acute rejection after lung transplantation is the main risk factor for the development of bronchiolitis obliterans (BO). Hemoglobin-vesicles (HbV) have been developed for use as artificial oxygen carriers and this can provide anti-inflammatory effects and may serve to limit tissue injury in airway transplant. Here, we tested the ability of HbV to prevent airway rejection. Tracheal grafts from BALB/c or C57BL/6 were transplanted to C57BL/6 recipients. Experimental groups were treated with HbV. Histopathological evaluation of luminal obliteration was blindly reviewed. Immunohistochemistry and real-time RT-PCR analyses were performed. Allografts treated with HbV revealed a reduction of thickening in epithelial and subepithelial airway layers at day 7 in orthotopic trachea transplantation model compared with allografts treated with vehicle. There was also a concordant decrease in CD3+ lymphocytes and macrophages in HbV treated allografts.
|
|
| Table of contents |
|
| Keyword |
|
| NDC |
|
| Note |
研究種目 : 若手研究(B)
研究期間 : 2012～2013
課題番号 : 24791470
研究分野 : 医歯薬学
科研費の分科・細目 : 外科系臨床医学 胸部外科学
|
|
| Language |
|
| Type of resource |
|
| Genre |
|
| Text version |
|
| Related DOI |
|
| Access conditions |
|
| Last modified date |
|
| Creation date |
|
| Registerd by |
|
| History |
|
| Index |
|
| Related to |
|
