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KAKEN_24657164seika.pdf
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Download
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:276.4 KB
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| Last updated |
:Apr 11, 2016 |
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Total downloads since Apr 11, 2016 : 1144
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| Title |
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ファイロティピック段階における形態の個体差とロバスト性
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ファイロティピック ダンカイ ニ オケル ケイタイ ノ コタイサ ト ロバストセイ
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Fairotipikku dankai ni okeru keitai no kotaisa to robasutosei
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Morphological diversity and robustness in phylotypic stage
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堀田, 耕司
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ホッタ, コウジ
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Hotta, Koji
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慶應義塾大学・理工学部・講師
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Research team head
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科研費研究者番号 : 80407147
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2015
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科学研究費補助金研究成果報告書
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2014
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カタユウレイボヤの未受精卵の一部を切除した卵片から矮小化(Dwarf)胚を作出する系を利用し, 発生過程において未受精卵の体積がその後の個体発生にどのように影響するのかを細胞レベルで調べた。その結果, Dwarf尾芽胚の組織ごとの細胞数は野生型(WT)尾芽胚と比較し, 減少する組織と一定である組織がある一方, 個体全体における組織ごとの体積比率はWTとDwarf間で一定であることが明らかとなった。すなわち, 卵の体積変化に関係なく, 組織ごとの体積を一定に保つ機構が存在することが示唆された。
Ascidian can produce normal-looking miniature tailbud embryos (dwarf embryo) even if unfertilized egg was partially removed. By using this dwarf embryo, we investigated how egg volume influenced on the afterward development at single cell level. As a result, it was found that several tissues (notochord, muscle and endoderm) kept the number of cells in dwarf embryo. On the other hand, other tissues (epidermis, neuron cells and mesenchyme) kept the size of each cell. However, the volume ratio of each tissue in whole-volume was same among dwarf and WT embryo. It was suggested that there is a robust mechanism to maintain a constant ratio of tissue volume regardless of egg volume.
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研究種目 : 挑戦的萌芽研究
研究期間 : 2012～2014
課題番号 : 24657164
研究分野 : 進化発生生物学
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