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KAKEN_23591516seika.pdf
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Star欠損マウスのステロイドホルモン産生細胞におけるトランスクリプトーム解析
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Star ケッソン マウス ノ ステロイド ホルモン サンセイ サイボウ ニ オケル トランスクリプトーム カイセキ
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Star kesson mausu no suteroido horumon sansei saibo ni okeru toransukuriputomu kaiseki
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Transcriptome analysis of steroidogenic cells in knockout mice lacking steroidogenic acute regulatory protein
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石井, 智弘
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イシイ, トモヒロ
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Ishii, Tomohiro
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慶應義塾大学・医学部・講師
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Research team head
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科研費研究者番号 : 70265867
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2014
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科学研究費補助金研究成果報告書
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2013
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先天性リポイド副腎過形成症(StAR異常症)の病態を解明するために、マウスStarのプロモーター、エンハンサー下にeGFPを発現する野生型(N=7)、Star欠損マウス(N=4)の胎生17.5-18.5日の副腎からeGFP陽性細胞を集積し、マイクロアレイで遺伝子発現を解析した。Star欠損マウスでは、1,206および767遺伝子の発現が上昇および低下し、コレステロール排出、炎症応答・免疫応答に関する遺伝子発現が有意に上昇し、副腎皮質にマクロファージが著明に浸潤していた。StAR欠損状態の副腎皮質において、コレステロール排出および炎症反応・免疫応答の亢進を初めて同定した。
Steroidogenic acute regulatory protein (StAR) is responsible for congenital lipoid adrenal hyperplasia (StAR deficiency). To delineate a specific pattern of gene expression associated with StAR deficiency, we analyzed the transcriptome of adrenocortical cells isolated by fluorescence-activated cell sorting at E17.5 or E18.5 from seven wild-type and four StAR knockout (Star-/-) mice with transgene targeting eGFP to StAR-expressing cell lineages. We identified that 1,206 and 767 genes were significantly up-regulated and down-regulated, respectively, and that genes involved in cholesterol efflux and inflammatory or immune response were significantly up-regulated in Star-/- mice. An increased number of macrophages were detected in the adrenal glands of Star-/- mice by immunohistochemistry. These results revealed the cholesterol efflux and the inflammatory or immune response as new pathophysiological hallmarks in the adrenocortical cells of Star-/- mice.
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研究種目 : 基盤研究(C)
研究期間 : 2011~2013
課題番号 : 23591516
研究分野 : 医歯薬学
科研費の分科・細目 : 内科系臨床医学・小児科学
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