広汎子宮頸部摘出術後妊娠（RT：25例）と子宮頸がん妊孕性温存手術を受けていない妊婦（コントロール：100例）を対象として、妊娠初期、中期、後期の3回腟分泌物を採取した。採取した腟分泌物は16SリボソーマルRNA解析を行い、網羅的な細菌叢を明らかにした。
3期間とも、乳酸桿菌の比率については両群間で有意差を認めなかった。一方で、コントロール群と比較して、RT群はα多様性が高いことが判明し、RT後妊娠では腟内細菌叢が変化することが早産の原因の一因になっている可能性が示唆された。
We enrolled pregnant women who had undergone radical trachcelectomy (RT: n=25) and those who had not undergone RT or cervical conization (control: n=100) and received perinatal care between 2019 and 2023. Vaginal discharge samples were collected during the first, second, and third trimesters and subjected to microbial analysis by sequencing the V1-V2 region of the 16S ribosomal RNA gene.
Throughout all trimesters, no differences in the incidence of Lactobacillus spp. were observed between the two groups. Compared with the control group, the RT group exhibited higher incidences of Streptococcus spp., Enterococcus spp., and Prevotella spp. in the first trimester; Prevotella spp. and Dialister spp. in the second trimester; and Streptococcus spp., Aerococcus spp., Prevotella spp., Dialister spp., and Mycoplasma spp. in the third trimester. Furthermore, the α-diversity analysis revealed alterations in the vaginal mycrobiota in the control group but not in the RT group.
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