レクチンマイクロアレイを用いた血清エクソソームの比較糖鎖解析を行い、ABA(Agaricus bisporus agglutinin)とACA(Amaranthus caudatus agglutinin)というO-型糖鎖を認識し結合するレクチンを同定した。同被験者の膵癌切除検体のパラフィン包埋切片をABA、ACAのレクチンを用いて染色したところ、膵癌の病変部および前癌病変部で染色陽性、正常膵組織は染色陰性であり膵癌特異的であることが確認され、新たな診断マーカーとなる可能性がある。本研究では予後が明らかになっている症例が膵癌68名のみであり、追跡期間も不十分であるため予後解析が行えていない。
We analyzed the dierential glycomic profiling of extracellular vesicles (EVs) derived from serum using lectin microarray. The glyco-candidates of PC-specific EVs were quantified using a high-sensitive exosome-counting system, ExoCounter. An absolute quantification system for altered glycan-containing EVs elevated in PC serum was established. EVs recognized by O-glycan-binding lectins ABA or ACA were identified as candidate markers by lectin microarray. Quantitative analyses using ExoCounter revealed that the ABA- or ACA-positive EVs were significantly increased in the culture of PC cell lines or in the serum of PC patients including carbohydrate antigen 19-9 negative patients with high area under curve values. Histological examination confirmed that the tumors stained with ABA/ACA. These specific EVs with O-glycans recognized by ABA/ACA are elevated in PC sera and can act as potential biomarkers in a liquid biopsy for PC patients screening.

研究種目 : 若手研究
研究期間 : 2019～2021
課題番号 : 19K18132
研究分野 : 膵癌Liquid Biopsy