好酸球性副鼻腔炎患者での50細胞でのRNA-seqの結果、末梢血と比べ鼻茸ILC2では2型サイトカイン、TNF, MAPK signaling pathwayに関連する遺伝子が高発現していた。LCI-S解析により2型サイトカインを高分泌するILC2が存在することが判明し、1細胞RNA-seq解析によりILC2の活性抑制候補遺伝子としてAnxa1を同定した。
喘息患者での検討では、末梢血から単離されるILC2数は健常人に比較し喘息患者で有意に少ないにも関わらず、1細胞レベルでのIL-5、IL-13分泌量は喘息患者由来のILC2で有意に高く、RNA-seqではIL-4受容体が高発現していた。
ILC2, isolated from nasal polyps and peripheral blood of patients with chronic rhinosinusitis, revealed the expression of Th2 cytokines and genes related to TNF and MAPK signaling pathways were upregulated in nasal polyps compared with peripheral blood by RNA-seq in 50 cells. Live cell imaging for secretion activity, LCI-S, revealed that there are the group of ILC2 that secret type 2 cytokines highly. Anxa1 was identified as a candidate gene that suppresses ILC2 activation by single cell RNA-seq analysis and ILC2 activation is regulated by Anxa1.
About asthma, the number of ILC2 was significantly lower in asthmatics than in healthy subjects. On the other hands, the amount of type 2 cytokines secretion by LCI-S, and the expression of IL-4 receptor by RNA-seq analysis were significantly higher in ILC2 from asthmatic patients. These results indicate that ILC2 from asthmatic patients has different cytokine production capacity and receptor expression.

研究種目 : 若手研究
研究期間 : 2019～2021
課題番号 : 19K17680
研究分野 : アレルギー