本研究で我々は、癌幹細胞やその癌幹細胞が生息する「立体的な癌幹細胞ニッチ」の解明に必要な研究基盤を整備した。①マウス腫瘍由来の「細胞単離→シングルセルRNAシークエンス」プロトコールは、ヒト腫瘍でも応用可能と考える。マウス腫瘍を最新のICELL8 cx Single-Cell System を利用し、8000を超える細胞のシークエンスを実装した、②独自のイメージング技術：DIIFCO法を臨床組織で実装し、癌幹細胞が生息するニッチ構造を3次元で可視化した、③多重免疫染色法を駆使した最新のsingle-cell pathologyで、臨床の腎細胞癌免疫微小環境を１細胞レベルで可視化した。
How to overcome the inter and intra "tumor heterogeneity" created by the cancer stemness and cellular hierarchy is an issue in recent cancer research. In this research, we have established a new research modality for elucidating cancer stem cells and the "three-dimensional cancer stem cell niche" in which they inhabit. First, single-cell isolation and RNA sequence protocol derived from mouse tumors can also be applied to human tumors; thereby using the latest ICELL8 cx Single-Cell System, we have implemented a sequence of over 8000 cells from mouse tumors. Second, using a new imaging platform named as the DIIFCO method and clinical tissues, we have uncovered the niche structure inhabited by cancer stem cells in three-dimension. Third, applying the latest multiplexed single-cell pathology, we have visualized the clinical renal cell carcinoma immune microenvironment at single-cell resolution. In sum, our imaging method may have a wider effect on a research community worldwide.

研究種目 : 基盤研究 (B) (一般)
研究期間 : 2019～2021
課題番号 : 19H03792
研究分野 : 泌尿器科学