Item Type |
Article |
ID |
|
Preview |
Image |
|
Caption |
|
|
Full text |
KAKEN_18K08600seika.pdf
Type |
:application/pdf |
Download
|
Size |
:433.2 KB
|
Last updated |
:Nov 14, 2022 |
Downloads |
: 159 |
Total downloads since Nov 14, 2022 : 159
|
|
Release Date |
|
Title |
Title |
ヒト由来幹細胞と脱細胞技術を用いた蠕動する機能的人工腸管作成の試み
|
Kana |
ヒト ユライ カンサイボウ ト ダツサイボウ ギジュツ オ モチイタ ゼンドウスル キノウテキ ジンコウ チョウカン サクセイ ノ ココロミ
|
Romanization |
Hito yurai kansaibō to datsusaibō gijutsu o mochiita zendōsuru kinōteki jinkō chōkan sakusei no kokoromi
|
|
Other Title |
Title |
Attempt to create a peristaltic functional artificial intestine using human-derived stem cells and decellularization technology
|
Kana |
|
Romanization |
|
|
Creator |
Name |
藤村, 匠
|
Kana |
フジムラ, タクミ
|
Romanization |
Fujimura, Takumi
|
Affiliation |
慶應義塾大学・医学部 (信濃町) ・助教
|
Affiliation (Translated) |
|
Role |
Research team head
|
Link |
科研費研究者番号 : 80573443
|
Name |
黄地, 健仁
|
Kana |
オウチ, タケヒト
|
Romanization |
Ōchi, Takehito
|
Affiliation |
慶應義塾大学・医学部 (信濃町) ・助教
|
Affiliation (Translated) |
|
Role |
Research team member
|
Link |
科研費研究者番号 : 30803564
|
Name |
黒田, 達夫
|
Kana |
クロダ, タツオ
|
Romanization |
Kuroda, Tatsuo
|
Affiliation |
慶應義塾大学・医学部 (信濃町) ・教授
|
Affiliation (Translated) |
|
Role |
Research team member
|
Link |
科研費研究者番号 : 60170130
|
Name |
芝田, 晋介
|
Kana |
シバタ, シンスケ
|
Romanization |
Shibata, Shinsuke
|
Affiliation |
慶應義塾大学・医学部 (信濃町) ・訪問教授
|
Affiliation (Translated) |
|
Role |
Research team member
|
Link |
科研費研究者番号 : 70407089
|
|
Edition |
|
Place |
|
Publisher |
|
Date |
Issued (from:yyyy) |
2021
|
Issued (to:yyyy) |
|
Created (yyyy-mm-dd) |
|
Updated (yyyy-mm-dd) |
|
Captured (yyyy-mm-dd) |
|
|
Physical description |
|
Source Title |
Name |
科学研究費補助金研究成果報告書
|
Name (Translated) |
|
Volume |
|
Issue |
|
Year |
2020
|
Month |
|
Start page |
|
End page |
|
|
ISSN |
|
ISBN |
|
DOI |
|
URI |
|
JaLCDOI |
|
NII Article ID |
|
Ichushi ID |
|
Other ID |
|
Doctoral dissertation |
Dissertation Number |
|
Date of granted |
|
Degree name |
|
Degree grantor |
|
|
Abstract |
短腸症候群や腸管運動不全に対する新規治療として、脱細胞化技術を用いて作製した腸管scaffoldにヒト歯髄細胞を用いて再細胞化を施し、機能的な人工小腸グラフトを構築することを目標とした。脱細胞技術によりラット小腸Scaffoldを作製でき、移植するヒト歯髄細胞の性質も確認できた。しかし、経血管循環培養(2週間)による再細胞化では移植細胞の多くが循環培養経路途中の腸間膜部分に留まり、免疫組織化学では腸管scaffold内の移植細胞を証明できず、電子顕微鏡でごく僅かに確認できたのみであった。ヒト細胞であるため、4週間の循環培養の延長も試みたが生着率向上にはつながらなかった。
As a new treatment for short bowel syndrome and intestinal dismotility, we aimed to construct a functional artificial small intestinal graft which made by decellularization technology and human dental pulp cells. A rat small intestine scaffold could be prepared by decellularization technology, and the properties of the human dental pulp cells to be transplanted could be confirmed. However, in recellarization by transvascular circulation culture (2 weeks), most of the transplanted cells stayed in the mesenterica in the circulation culture route. Immunohistochemistry could not prove the transplanted cells in the intestinal scaffold. Only a few cells could be confirmed by scanning electron microscope. We tried to extend the circulation culture for 4 weeks, but it did not lead to an improvement in the engraftment rate.
|
|
Table of contents |
|
Keyword |
|
NDC |
|
Note |
研究種目 : 基盤研究 (C) (一般)
研究期間 : 2018~2020
課題番号 : 18K08600
研究分野 : 腸管運動不全
|
|
Language |
|
Type of resource |
|
Genre |
|
Text version |
|
Related DOI |
|
Access conditions |
|
Last modified date |
|
Creation date |
|
Registerd by |
|
History |
May 17, 2022 | | インデックス を変更 |
Nov 14, 2022 | | Creator 著者ID,Creator Name,Creator Kana,Creator Romanization,Creator Affiliation,Creator Affiliation (Translated),Creator Role,Creator Link,Abstract 内容,Note 注記 を変更 |
|
|
Index |
|
Related to |
|