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KAKEN_17K15129seika.pdf
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:80.4 KB
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:Oct 31, 2019 |
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Total downloads since Oct 31, 2019 : 263
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| Title |
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ヒトES細胞の分化過程における転写因子ネットワークの解明と新規分化誘導因子の同定
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ヒト ES サイボウ ノ ブンカ カテイ ニ オケル テンシャ インシ ネットワーク ノ カイメイ ト シンキ ブンカ ユウドウ インシ ノ ドウテイ
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Hito ES saibō no bunka katei ni okeru tensha inshi nettowāku no kaimei to shinki bunka yūdō inshi no dōtei
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Elucidating of transcription factor network during the differentiation of human ES cells
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秋山, 智彦
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アキヤマ, トモヒコ
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Akiyama, Tomohiko
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| Affiliation |
慶應義塾大学・医学部 (信濃町)・助教
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Research team head
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科研費研究者番号 : 20570691
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2019
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科学研究費補助金研究成果報告書
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2018
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| Abstract |
転写因子導入によるヒトES細胞の分化誘導を制御するオープンクロマチンを同定するためにATACシーケンス法を行った。MYOD1導入による骨格筋分化およびNGN2導入による神経分化の解析を行い、それぞれに特異的に存在するオープンクロマチン領域を数千箇所同定した。さらに発生過程や成体の細胞において元来MYOD1やNGN2が結合するオープンクロマチン領域以外に、ES細胞に導入した際に特異的に出現するオープンクロマチン領域が存在することを明らかになった。これら2タイプのオープンクロマチン領域により制御される近傍遺伝子が協調して働くことによって、ダイレクトな細胞性質変換が可能になると考えられる。
ATAC sequencing was performed to identify open chromatin that regulates differentiation of human ES cells by introduction of transcription factors. Skeletal muscle differentiation by introducing MYOD1 and neuronal differentiation by introducing NGN2 was analysed and we identified several thousands of open chromatin sites specifically present in the differentiated cells. In addition to the sites that MYOD1 and NGN2 originally bind to in adult tissues, there were open chromatin sites that appear specifically when MYOD1 and NGN2 were introduced into ES cells. Together, direct conversions of ES cells to terminally differentiated cells can be achieved by the coordinated action controlled by these two types of open chromatin sites.
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研究種目 : 若手研究(B)
研究期間 : 2017～2018
課題番号 : 17K15129
研究分野 : 転写制御
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